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Quorum sensing systems of regulation, synthesis of phenazine antibiotics, and antifungal activity in rhizospheric bacterium pseudomonas chlororaphis 449

  • Genetics of Microorganisms
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An Erratum to this article was published on 18 September 2009

Abstract

Strain Pseudomonas chlororaphis 449, an antagonist of a broad spectrum of phytopathogenic microorganisms isolated from the maize rhizosphere, was shown to produce three phenazine antibiotics: phenazine-1-carboxylic acid (PCA), 2-hydroxylphenazine-1-carboxylic acid (2-OH-PCA), and 2-hydroxylphenazine (2-OH-PHZ). Two Quorum Sensing (QS) systems of regulation were identified: Phz/R and CsaI/R. Genes phzI and csaI were cloned and sequenced. Cells of strain 449 synthesize at least three types of AHL: N-butanoyl-L-homoserine lactone (C4-AHL), N-hexanoyl-L-homoserine lactone (C6-AHL), and N-(3-oxo-hexanoyl)-L-homoserine lactone (30C6-AHL). Transposon mutagenesis was used to generate mutants of strain 449 deficient in synthesis of phenazines, which carried inactivated phzA and phzB genes of the phenazine operon and gene phzO. Mutations phzA − and phzB − caused a drastic reduction in the antagonistic activity of bacteria toward phytopathogenic fungi. Both mutants lost the ability to protect cucumber and leguminous plants against phytopathogenic fungi Rhizoctonia solani and Sclerotinia sclerotiorum. These results suggest a significant role of phenazines in the antagonistic activity of P. chlororaphis 449.

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Authors and Affiliations

  1. Institute of Molecular Genetics, Russian Academy of Sciences, Moscow, 123182, Russia

    M. A. Veselova, I. A. Bass, V. A. Lipasova, A. Z. Metlitskaya & I. A. Khmel

  2. Otto Warburg Center for Biotechnology, Faculty of Agricultural, Food and Environmental Quality Sciences, Hebrew University of Jerusalem, Rehovot, 76100, Israel

    Sh. Klein, M. I. Ovadis & L. S. Chernin

Authors
  1. M. A. Veselova
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  2. Sh. Klein
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  3. I. A. Bass
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  4. V. A. Lipasova
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  5. A. Z. Metlitskaya
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  6. M. I. Ovadis
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  7. L. S. Chernin
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  8. I. A. Khmel
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Corresponding author

Correspondence to I. A. Khmel.

Additional information

Original Russian Text © M.A. Veselova, Sh. Klein, I.A. Bass, V.A. Lipasova, A.Z. Metlitskaya, M.I. Ovadis, L.S. Chernin, I.A. Khmel, 2008, published in Genetika, 2008, Vol. 44, No. 12, pp. 1617–1626.

An erratum to this article can be found at http://dx.doi.org/10.1134/S1022795409090178

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Veselova, M.A., Klein, S., Bass, I.A. et al. Quorum sensing systems of regulation, synthesis of phenazine antibiotics, and antifungal activity in rhizospheric bacterium pseudomonas chlororaphis 449. Russ J Genet 44, 1400–1408 (2008). https://doi.org/10.1134/S102279540812003X

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