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Impact of Bacillus subtilis Bacteria in Combination with Salicylic and Jasmonic Acids on Changing the Proteome of Potato Leaves when Infected by Phytophthora infestans (Mont.) De Bary and with a Moisture Deficit

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Abstract

The effect of Bacillus subtilis bacteria in combination with salicylic (SA) and jasmonic (JA) acids on changes in the proteome of potato leaves during infection with Phytophthora infestans (Mont.) de Bary and moisture deficit was studied. Plants grown from microtubers of the Rannyaya Roza variety were sprayed with a suspension of B. subtilis (108 cells/mL) and a mixture of bacteria with SA (10–6 M), JA (10–7 M), and SA + JA. The plants were infected with P. infestans (105 spores/mL) 3 days after treatment and cultivated under conditions of artificial soil drought by reducing watering. When the soil moisture reached 40 ± 5% of the total water capacity (7 days after infection), the plants were fixed in liquid nitrogen to isolate proteins and analyze them by two-dimensional electrophoresis and mass spectrometry. A decrease in the degree of infestation with P. infestans was shown when potato leaves were treated with B. subtilis in combination with SA and JA. The leaf proteome shows differences in the content of 14 proteins in the pI range from 4.0 to 9.0 with molecular masses from 30 to 125 kDa. The most significant changes in the spectrum of plant proteins were found in healthy plants treated with B. subtilis and in infected plants in the combination of B. subtilis with JA. Qualitative and quantitative changes were observed for proteins involved in the processes of respiration and hypersensitivity reactions (HSR), energy metabolism, synthesis of secondary metabolites, and protective proteins that affect plant resistance to abiotic and biotic stress. Proteomic analysis has identified important proteins involved in the mechanism of potato responses to treatment with B. subtilis and infection with P. infestans, although the exact functions of the identified proteins and their potential impact on potato plant resistance to P. infestans and moisture deficit remain to be elucidated.

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Funding

The work was partly carried out on the topic of the State Assignment (state registration number AAAA-A21-121011990120-7), with the financial support of the Russian Foundation for Basic Research and the Belarusian Republican Foundation for Basic Research within the framework of scientific project no. 20-516-00005, using the equipment of the Center for Collective using the “Human Proteome” (IBMC).

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Authors and Affiliations

  1. Institute of Biochemistry and Genetics, Ufa Federal Research Center, Russian Academy of Sciences, Ufa, Russia

    L. G. Yarullina, E. A. Cherepanova, G. F. Burkhanova & E. A. Zaikina

  2. Federal State Budgetary Educational Institution of Higher Education Bashkir State University, Ufa, Russia

    V. O. Tsvetkov & V. O. Khabibullina

  3. Institute of Experimental Botany named after V.F. Kuprevich, National Academy of Sciences of Belarus, Minsk, Belarus

    J. N. Kalatskaya

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  1. L. G. Yarullina
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  2. V. O. Tsvetkov
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  4. E. A. Cherepanova
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  5. G. F. Burkhanova
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Correspondence to L. G. Yarullina.

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Translated by M. Shulskaya

Abbreviations: JA—jasmonic acid; ISR—induced systemic resistance; SA—salicylic acid; SOD—superoxide dismutase; PGSB—plant growth stimulating bacteria; SAR—systemic acquired resistance; GCS—glycine cleavage system; GDC—glycine dehydrogenase (decarboxylating); mARK—mitochondrial amidoxime reducing component; OEEP—oxygen-evolving enhancer protein; RBOH—respiratory burst oxidase homologs; SHMT—serine hydroxymethyltransferase.

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Yarullina, L.G., Tsvetkov, V.O., Khabibullina, V.O. et al. Impact of Bacillus subtilis Bacteria in Combination with Salicylic and Jasmonic Acids on Changing the Proteome of Potato Leaves when Infected by Phytophthora infestans (Mont.) De Bary and with a Moisture Deficit. Russ J Plant Physiol 69, 81 (2022). https://doi.org/10.1134/S1021443722040215

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