Skip to main content
SpringerLink
Log in

Effect of salicylic acid and yeast extract on the accumulation of jasmonic acid and sesquiterpenoids in Panax ginseng adventitious roots

  • Research Papers
  • Published:
Russian Journal of Plant Physiology Aims and scope Submit manuscript

Abstract

In different plant species, secondary metabolite biosynthesis is regulated by the phytohormone jasmonic acid (JA), which is derived by the action of lipoxygenase. In this study, we examined mono- and sesquiterpenoid accumulation and the related signal transduction pathways and biosynthetic genes in adventitious root cultures of Panax ginseng C.A. Meyer as induced by yeast extract (YE, 3 g/L), a biotic elicitor, and salicylic acid (SA, 200 μM), a signaling elicitor. The lipoxygenase (LOX) gene was highly expressed in 24 and 12 h after treatment with SA and YE. JA content was significantly increased in 24 h after SA treatment. The H2O2 content was the highest in 24 and 72 h after the onset of SA and YE treatment, respectively. RNA blot analysis showed that farnesyl diphosphate synthase (FPS) and isopentenyl pyrophosphate isomerase (IPPI) genes encoding enzymes of the biosynthesis of mono- and sesquiterpenoids were up-regulated by both elicitors. Farensol, isochiapin B sesquiterpenoids, champhor, and cineole monoterpenoids were highly accumulated after 24 h of SA treatment, while YE treatment induced bacchotricuneatin C, guaiazulene, isochiapin B, and p-benzoquinone sesquiterpenoid production. These results suggest that mono- and sesquiterpenoid accumulation induced by SA and YE occurs due to the IPPI and FPS expression and may be mediated by reactive oxygen species signaling and jasmonic acid signal transduction.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

DW:

dry weight

FPS:

farnesyl diphosphate synthase

FW:

fresh weight

IPPI:

isopentenyl pyrophosphate isomerase

JA:

jasmonic acid

LOX:

lipoxygenase

SA:

salicylic acid

YE:

yeast extract

References

  1. Montesano, M., Brader, G., and Palva, E.T., Pathogen-derived elicitors: searching for receptors in plants, Mol. Plant Pathol., 2003, vol. 4, pp. 73–79.

    Article  PubMed  CAS  Google Scholar 

  2. Vasconsuelo, A. and Boland, R., Molecular aspects of the early stages of elicitation of secondary metabolites in plants, Plant Sci., 2007, vol. 172, pp. 861–875.

    Article  CAS  Google Scholar 

  3. Zhao, J., Lawrence, C., Davis, T., and Verpoorte, R., Elicitor signal transduction leading to production of plant secondary metabolites, Biotechnol. Adv., 2005, vol. 23, pp. 283–333.

    Article  PubMed  CAS  Google Scholar 

  4. Cragg, G.M., Paclitaxel (taxol): a success story with valuable lessons for natural product drug discovery and development, Med. Res. Rev., 1998, vol. 18, pp. 315–331.

    Article  PubMed  CAS  Google Scholar 

  5. Dhingra, V., Rao, K.V., and Narasu, M.L., Current status of artemisinin and its derivatives as antimalarial drugs, Life Sci., 2000, vol. 66, pp. 279–300.

    Article  PubMed  CAS  Google Scholar 

  6. Lichtenthaler, H.K., Schwender, J., Disch, A., and Rohmer, M., Biosynthesis of isoprenoids in higher plant chloroplasts proceeds via a mevalonate-independent pathway, FEBS Lett., 1997, vol. 400, pp. 271–274.

    Article  PubMed  CAS  Google Scholar 

  7. Murashige, T. and Skoog, F., A revised medium for rapid growth and bioassay with tobacco tissue cultures, Plant Physiol., 1962, vol. 15, pp. 473–499.

    Article  CAS  Google Scholar 

  8. Liu, X., Yang, Y.L., Lin, W.H., Tong, J.H., Huang, Z.G., and Xiao, L.T., Determination of both jasmonic acid and methyl jasmonate in plant samples by liquid chromatography tandem mass spectrometry, Chinese Sci. Bull., 2010, vol. 55, pp. 2231–2235.

    Article  CAS  Google Scholar 

  9. Brennan, T. and Frenkel, C., Involvement of hydrogen peroxide in regulation of senescence in pear, Plant Physiol., 1977, vol. 59, pp. 411–416.

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  10. Lee, S. and Chappell, J., Biochemical and genomic characterization of terpene synthases in Magnolia grandiflora, Plant Physiol., 2008, vol. 147, pp. 1017–1033.

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  11. Gundlach, H., Muller, M.J., Kutchan, T.M., and Zenk, M.H., Jasmonic acid is a signal transducer in elicitor-induced plant cell cultures, Proc. Natl. Acad. Sci. USA, 1992, vol. 89, pp. 2389–2393.

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  12. Khalili, M., Hasanloo, T., Kazemi, Tabar, S.K., and Rahnama, H., Influence of exogenous salicylic acid on flavonolignans and lipoxygenase activity in the hairy root cultures of Silybum marianum, Cell Biol. Int., 2009, vol. 33, pp. 988–994.

    Article  PubMed  CAS  Google Scholar 

  13. Hasanloo, T., Sepehrifar, R., Rahnama, H., and Shams, M., Evaluation of the yeast-extract signaling pathway leading to silymarin biosynthesis in milk thistle hairy root culture, J. Microbiol. Biotechnol., 2009, vol. 25, pp. 1901–1909.

    Article  CAS  Google Scholar 

  14. Degousee, N., Triantaphylides, C., and Montillet, J.L., Involvement of oxidative processes in the signaling mechanisms leading to the activation of glyceollin synthesis in soybean (Glycine max), Plant Physiol., 1994, vol. 104, pp. 945–952.

    PubMed  CAS  PubMed Central  Google Scholar 

  15. Matsuda, F., Miyagawa, H., and Ueno, T., Involvement of reactive oxygen species in the induction of (S)-N-p-coumaroyloctopamine accumulation by β-1,3-glucooligosaccharide elicitors in potato tuber tissues, J. BioSci., 2001, vol. 56, pp. 228–234.

    CAS  Google Scholar 

  16. Zhao, J., Hu, Q., Guo, Y.Q., and Zhu, W.H., Elicitorinduced indole alkaloid biosynthesis in Catharanthus roseus cell cultures is related to Ca2+-influx and the oxidative burst, Plant Sci., 2001, vol. 161, pp. 423–431.

    Article  CAS  Google Scholar 

  17. Kravchuk, Zh.M., Perkovs’ka, H.Yu., and Dmytriiev, O.P., Role of active forms of oxygen in the induction of phytoalexin synthesis in Allium cepa cells, Tsitol. Genet., 2003, vol. 37, pp. 30–35.

    PubMed  CAS  Google Scholar 

  18. Hu, X., Neill, S.J., Cai, W., and Tang, Z., Hydrogen peroxide and jasmonic acid mediate oligogalacturonic acid-induced saponin synthesis in suspension-cultured cells of Panax ginseng, Physiol. Plant., 2003, vol. 118, pp. 414–421.

    Article  CAS  Google Scholar 

  19. Zhao, J. and Sakai, K., Peroxidases are involved in the biosynthesis and biodegradation of β-thujaplicin in fungal elicitor-treated Cupressus lusitanica suspension cultures, New Phytol., 2003, vol. 159, pp. 719–731.

    Article  CAS  Google Scholar 

  20. Wibberley, M.S., Lenton, J.R., and Neill, S.J., Sesquiterpenoid phytoalexins produced by hairy roots of Nicotiana tabacum, Phytochemistry, 1994, vol. 37, pp. 349–351.

    Article  CAS  Google Scholar 

  21. Singh, G., Gavriel, J., Oakey, J.S., and Curtis, W.R., Interaction of methyl jasmonate, wounding and fungal elicitation during sequiterpene induction in Hyoscyamus muticus in root cultures, Plant Cell Rep., 1998, vol. 17, pp. 391–395.

    Article  CAS  Google Scholar 

  22. Khorolragchaa, A., Parvin, S., Shim, J.S., Kim, Y.J., Lee, O.R., In, J.G., Kim, Y.J., Kim, S.Y., and Yang, D.C., Isolation of sesquiterpene synthase homolog from Panax ginseng C.A. Meyer, J. Ginseng Res., 2010, vol. 34, pp. 17–22.

    Article  CAS  Google Scholar 

  23. Kittipongpatana, N., Davis, D.L., and Porter, J.R., Methyl jasmonate increases the production of valepotriates by transformed root cultures of Valerianella locusta, Plant Cell Tissue Organ Cult., 2002, vol. 71, pp. 65–75.

    Article  CAS  Google Scholar 

  24. Wielanek, M. and Urbanek, H.K., Enhanced glucotropaeolin production in hairy root cultures of Tropaeolum majus L. by combining elicitation and precursor feeding, Plant Cell Tissue Organ Cult., 2006, vol. 86, pp. 177–186.

    Article  CAS  Google Scholar 

  25. Mehmetoglu, U. and Curtis, W.R., Effects of abiotic inducers on sesquiterpene synthesis in hairy root and cell-suspension cultures of Hyoscyamus muticus, Appl. Biochem. Biotechnol., 1997, vol. 67, pp. 71–77.

    Article  CAS  Google Scholar 

  26. Chen, H. and Chen, F., Effect of yeast elicitor on the secondary metabolism of Ti-transformed Salvia miltiorrhiza cell suspension cultures, Plant Cell Rep., 2000, vol. 19, pp. 710–717.

    Article  CAS  Google Scholar 

  27. Chen, D.H., Ye, H.C., and Li, G.F., Expression of a chimeric farnesyl diphosphate synthase gene in Artemisia annua L. transgenic plants via Agrobacterium tumefaciens-mediated transformation, Plant Sci., 2000, vol. 155, pp. 179–185.

    Article  PubMed  CAS  Google Scholar 

  28. Taguchi, G., Yazawa, T., Hayashida, N., and Okazaki, M., Molecular cloning and heterologous expression of novel glucosyltransferases from tobacco cultured cells that have broad substrate specificity and are induced by salicylic acid and auxin, Eur. J. Biochem., 2001, vol. 268, pp. 4086–4094.

    Article  PubMed  CAS  Google Scholar 

  29. Kang, S.M., Jung, H.Y., Kang, Y.M., Yun, D.J., Bahk, J.D., Yang, J., and Choi, M.S., Effects of methyl jasmonate and salicylic acid on the production of tropane alkaloids and the expression of PMT and H6H in adventitious root cultures of Scopolia parviflora, Plant Sci., 2004, vol. 166, pp. 745–751.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Oriental Medicinal Materials and Processing and Ginseng Genetic Resource Bank, Kyung Hee University, 1 Seocheon, Giheung-gu Yongin-si, Gyeonggi-do, 449-701, South Korea

    S. Rahimi, B. S. R. Devi, A. Khorolragchaa, Y. J. Kim, J. H. Kim, S. K. Jung & D. C. Yang

Authors
  1. S. Rahimi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. B. S. R. Devi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. Khorolragchaa
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Y. J. Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. J. H. Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. S. K. Jung
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. D. C. Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to D. C. Yang.

Additional information

This text was submitted by the authors in English.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Rahimi, S., Devi, B.S.R., Khorolragchaa, A. et al. Effect of salicylic acid and yeast extract on the accumulation of jasmonic acid and sesquiterpenoids in Panax ginseng adventitious roots. Russ J Plant Physiol 61, 811–817 (2014). https://doi.org/10.1134/S1021443714060156

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S1021443714060156

Keywords

Search

Navigation