Abstract
Proteins diffusing from tobacco pollen grains into external medium, being inactivated by low temperature (0°C), were shown to stimulate pollen germination in vitro. Fractionation of these proteins by affinity chromatography using α-D-methylmannopyranoside (MMP) immobilized on agarose resulted in the isolation of lectins stimulating germination. The mol wts of these lectins were estimated by SDS-PAGE as 58, 69, and 74 kD. A stimulatory effect of these lectins was determined by their specific interaction with carbohydrate determinants because a competitive sugar (0.3 M MMP) suppressed completely lectin effect on germination. Polyvalent lectins capable of erythrocyte agglutination were also found among diffused proteins. These lectins are glycoproteins with Glu/Man carbohydrate determinants. MMP did not affect their capability of agglutination. This finding permits a conclusion that pollen grain wall contains lectins differing in their carbohydrate specificity.
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Abbreviations
- ConA:
-
concavalin A
- MMP:
-
α-D-methylmannopyranoside (MMP)
References
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Original Russian Text © N.P. Matveeva, E.A. Lazareva, T.P. Klyushnik, S.A. Zozulya, I.P. Ermakov, 2007, published in Fiziologiya Rastenii, 2007, Vol. 54, No. 5, pp. 699–706.
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Matveeva, N.P., Lazareva, E.A., Klyushnik, T.P. et al. Lectins of the Nicotiana tabacum pollen grain walls stimulating in vitro pollen germination. Russ J Plant Physiol 54, 619–625 (2007). https://doi.org/10.1134/S1021443707050081
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DOI: https://doi.org/10.1134/S1021443707050081