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Inhibitory effect of fucoidan from brown alga Fucus evanescens on the spread of infection induced by tobacco mosaic virus in tobacco leaves of two cultivars

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Abstract

The effect of fucoidan from the brown alga Fucus evanescens on the spread of infection induced by tobacco mosaic virus (TMV) was investigated in the leaves of tobacco (Nicotiana tabacum L.) of two cultivars (Ksanti-nk and Samsun). In the leaves of cv. Ksanti-nk inoculated with a mixture of TMV preparation (2 μg/ml) and fucoidan (1 mg/ml), the number of local necrotic lesions induced by the virus decreased by more than 90% as compared with the leaves inoculated with the virus alone. In tobacco leaves of cv. Samsun, virulence and the concentration of the virus 3 days after inoculation with the same mixture of TMV and fucoidan were by 62 and 66%, respectively, lower than in the leaves inoculated with TMV alone. As the infection spread, the inhibitory effect of fucoidan decreased. When the leaves were treated with fucoidan before and after the inoculation with TMV, its antiviral activity was less pronounced than when a mixture of the virus and the polysaccharide was used as inoculum. Electron microscopic investigation of TMV mixed with fucoidan often showed agglutinated virions. The highest virulence of the mixture (TMV preparation, 12 μg/ml, plus fucoidan, 1 mg/ml) was observed upon its twofold dilution, and after that it decreased. It was concluded that, when the leaves were inoculated with the mixture of TMV and fucoidan, the latter affected not only the plant but the virus as well. Treatment of tobacco leaves, cv. Ksanti-nk, with actinomycin D (10 μg/ml) 24 h before the inoculation with TMV almost completely suppressed the effect of fucoidan, indicating that fucoidan acted at a gene level.

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Abbreviations

AMD:

actinomycin D

PTA:

phosphotungstic acid

TMV:

tobacco mosaic virus

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Authors and Affiliations

  1. Pacific Institute of Bioorganic Chemistry, Far East Division, Russian Academy of Sciences, pr. Stoletiya Vladivostoka 159, Vladivostok, 690022, Russia

    L. A. Lapshina, A. V. Reunov, V. P. Nagorskaya, T. N. Zvyagintseva & N. M. Shevchenko

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  1. L. A. Lapshina
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  2. A. V. Reunov
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  3. V. P. Nagorskaya
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  4. T. N. Zvyagintseva
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  5. N. M. Shevchenko
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Correspondence to L. A. Lapshina.

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Original Russian Text © L.A. Lapshina, A.V. Reunov, V.P. Nagorskaya, T.N. Zvyagintseva, N.M. Shevchenko, 2006, published in Fiziologiya Rastenii, 2006, Vol. 53, No. 2, pp. 274–279.

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Lapshina, L.A., Reunov, A.V., Nagorskaya, V.P. et al. Inhibitory effect of fucoidan from brown alga Fucus evanescens on the spread of infection induced by tobacco mosaic virus in tobacco leaves of two cultivars. Russ J Plant Physiol 53, 246–251 (2006). https://doi.org/10.1134/S1021443706020154

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  • DOI: https://doi.org/10.1134/S1021443706020154

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