Skip to main content
Log in

Expression of stress response genes in barley Hordeum vulgare in a spaceflight environment

  • Genomics and Transcriptomics
  • Published:
Molecular Biology Aims and scope Submit manuscript

Abstract

The transcriptome of barley Hordeum vulgare grown aboard the International Space Station was studied using microarray analysis. In the spaceflight environment, mRNA levels of over 500 genes were changed more than twofold; among them, genes of stress response proteins, in particular, heat shock proteins, pathogenesis-related proteins, and antioxidant proteins. Further analysis by real-time PCR confirmed enhanced transcription of reactive oxygen species scavenging genes. The superoxide dismutase (sod) mRNA level in the space environment was 6-fold higher than in earth conditions. The transcript levels of glutamyl transferase (gst), catalase (cat), and ascorbate peroxidase (apx) were increased in spaceflight 24, 18, and 3 times in comparison to ground control, respectively. For the first time, it has been shown that spaceflight environment can induce oxidative stress in plants.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Souza K.A., Ilyin E.A., Sychev V.N., Jahns G. C. 2009. In: Space Biology and Medicine. U.S. and Russian Cooperation in Space Biology and Medicine. Joint U.S.-Russian Publication. Reston, Virginia: AIAA, chapter 1, pp. 1–43.

    Google Scholar 

  2. Levinskikh M.A., Sychev V.N., Derendyaeva T.A., Signalova O.B., Salisbury F.B., Campbell W.F., Bingham G.E., Bubenheim D.L., Jahns G. 2000. Analysis of the space-flight effects on growth and development of super dwarf wheat grown on the Space Station Mir. J. Plant Physiol. 156, 522–529.

    CAS  PubMed  Google Scholar 

  3. Sychev V.N., Levinskikh M.A., Podolsky I.G., Novikova N.D., Gostimsky S.A., Alekseev V.A., Bingham G. 1994. Main results of experiments on higher plants and dormant forms of organisms onboard the Russian segment of the International Space Station. Kosmonavtika i Raketostroenie. 49, 54–64.

    Google Scholar 

  4. Gostimsky S.A., Levinskikh M.A., Sychev V.N., Kokaeva Z.G., Dribnokhodova O.P., Khartina G.A., Bingham G. 2007. The Study of the Genetic Effects in Generation of Pea Plants Cultivated During the Whole Cycle of Ontogenesis on the Board of RS ISS. Genetika. 43, 1050–1057.

    Google Scholar 

  5. Levinskikh M.A., Sychev V.N., Derendyaeva T.A., Signalova O.B., Podolsky I.G., Gostimsky S.A., Bingham G. 2005. Characteristics of growth, development, and genetic status of pea plants grown in the Lada space greenhouse. Aviakosm. Ekol. Med. 39, 38–43.

    CAS  Google Scholar 

  6. Levinskikh M.A., Sychev V.N., Signalova O.B., Derendyaeva T.A., Nefedova E.L., Musgrave M.E., Campbell U.F., J., Konlin D., Bingham G.E. 2002. Some characteristics of plant seeds formed under conditions of microgravity. Aviakosm. Ekol. Med. 36, 62–64.

    Google Scholar 

  7. http://www3.appliedbiosystems.com/cms/grou…/cms-042380.pdf.

  8. Escherichia coli and Salmonella. Cellular and Molecular Biology. 1996. Ed. Neighart F.C. Washington: ASM Press.

    Google Scholar 

  9. Hartman D.J., Dougan D., Hoogenraad N.J., Hoj P.B. 1992. Heat shock proteins of barley mitochondria and chloroplasts. Identification of organellar hsp 10 and 12: Putative chaperonin 10 homologues. FEBS Lett. 305, 147–150.

    Article  CAS  PubMed  Google Scholar 

  10. Shilova V.Yu., Garbuz D.G., Evgen’ev M.B., Zatsepina O.G. 2006. Small heat shock proteins and adaptation of various Drosophila species to hyperthermia. Mol. Biol. 40, 235–249.

    Article  CAS  Google Scholar 

  11. Esfandiari E., Shekari F., Shekari F., Esfandiari M. 2007. The effect of salt stress on antioxidant enzymes’ activity and lipid peroxidation on the wheat seedling. Not. Bot. Hort. Agrobot. Cluj. 35, 48–56.

    CAS  Google Scholar 

  12. Langridge P., Paltridge N., Fincher G. 2006. Functional genomics of abiotic stress tolerance in cereals. Brief. Funct. Genomic Proteomic.4, 343–354.

    Article  CAS  PubMed  Google Scholar 

  13. Hammond T.G., Benes E., O’Reilly K.C., Wolf D.A., Linnehan R.M., Taher A., Kaysen J.H., Allen P.L., Goodwin T.J. 2000. Mechanical culture conditions effect gene expression: Gravity-induced changes on the space shuttle. Physiol. Genomics. 10, 163–173.

    Google Scholar 

  14. Paul A.-L., Popp M.P., Gurley W.B., Guy C., Norwood K.L., Ferl R.J. 2005. Arabidopsis gene expression patterns are altered during spaceflight. Adv. Space Res. 36, 1175–1181.

    Article  Google Scholar 

  15. Martzivanou M., Hampp R. 2003. Hyper-gravity effects on the Arabidopsis transcriptome. Physiol. Plantarum. 118, 221–231.

    Article  CAS  Google Scholar 

  16. Kozeko L., Kordyum E. 2008. Effect of hypergravity on the level of heat shock proteins 70 and 90 in pea seedlings. Microgravity — Science and Technology. Published online.

  17. Yun H.K., Yi S.Y., Yu S.H., Choi D. 1999. Cloning of a pathogenesis-related protein-1 gene from Nicotiana glutinosa L. and its salicylic acid-independent induction by copper and β-aminobutyric acid. J. Plant Physiol. 154, 327–333.

    CAS  Google Scholar 

  18. Pääkkönen E., Seppänen S., Holopainen T., Kokko H., Kärenlampi S., Kärenlampi L., Kangasjärvi J. 1998. Induction of genes for the stress proteins PR-10 and PAL in relation to growth, visible injuries and stomatal conductance in birch (Betula pendula) clones exposed to ozone and/or drought. New Phytol. 138, 295–305.

    Article  Google Scholar 

  19. Seo P.J., Lee A.K., Xiang F., Park C.-M. 2008. Molecular and functional profiling of Arabidopsis pathogenesis-related genes: Insights into their roles in salt response of seed germination. Plant Cell Physiol. 49, 334–344.

    Article  CAS  PubMed  Google Scholar 

  20. Li G., Liu Y., Wang G., Song L. 2004. Reactive oxygen species and antioxidant enzymes activity of Anabaena sp. PCC 7120 (Cyanobacterium) under simulated microgravity. Acta Astronautica. 55, 953–957.

    CAS  PubMed  Google Scholar 

  21. Horneck G. 1992. Radiobiological experiments in space: A review. Int. J. Radiat. Appl. Instrum. 20, 185–205.

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to E. I. Shagimardanova.

Additional information

Original Russian Text © E.I. Shagimardanova, O.A. Gusev, V.N. Sychev, M.A. Levinskikh, M.R. Sharipova, O.N. Il’inskaya, G. Bingham, M. Sugimoto, 2010, published in Molekulyarnaya Biologiya, 2010, Vol. 44, No. 5, pp. 831–838.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Shagimardanova, E.I., Gusev, O.A., Sychev, V.N. et al. Expression of stress response genes in barley Hordeum vulgare in a spaceflight environment. Mol Biol 44, 734–740 (2010). https://doi.org/10.1134/S0026893310050080

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S0026893310050080

Key words

Navigation