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Ultrastructural and Functional Changes in Liver Mitochondria in a Rat Model of Type I Diabetes Mellitus

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Abstract—The characteristics of the ultrastructure and functioning of mitochondria in the liver of Sprague Dawley rats in the experimental model of type I diabetes mellitus have been investigated. It has been found that diabetes mellitus induced in response to streptozotocin administration at a dose of 75 mg/kg body weight was accompanied by disturbances in the structural organization of mitochondrial cristae and a decrease in the size of the organelles compared to the control. It has been also demonstrated that in type I diabetes the respiratory rates of liver mitochondria in metabolic states 2, 3, and 4 increase. This may be associated with an increase in the total content of fatty acids in liver mitochondria of diabetic rats. At the same time, streptozotocin-induced diabetes mellitus in rats did not affect the indices of oxidative phosphorylation efficiency (ADP/O, respiratory control ratio, and phosphorylation time) in liver mitochondria.

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Funding

The work was supported by the Russian Foundation for Basic Research, project no. 19-015-00117-а.

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Authors and Affiliations

  1. Mari State University, 424000, Yoshkar-Ola, Russia

    V. S. Starinets, E. V. Lebedeva, M. V. Dubinin & K. N. Belosludtsev

  2. Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, 142290, Pushchino, Moscow oblast, Russia

    I. B. Mikheeva, N. V. Belosludtseva & K. N. Belosludtsev

Authors
  1. V. S. Starinets
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  2. E. V. Lebedeva
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  3. I. B. Mikheeva
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  4. N. V. Belosludtseva
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  5. M. V. Dubinin
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  6. K. N. Belosludtsev
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Corresponding author

Correspondence to V. S. Starinets.

Ethics declarations

The study was performed in accordance with the European Convention for the protection of vertebrate animals used for experimental and other scientific purposes (Strasbourg, 1986) and the ethical principles of the Declaration of Helsinki (2000). All the experimental protocols were approved by the Ethics Committee of the Institute of Theoretical and Experimental Biophysics of the Russian Academy of Sciences (order no. 173 / from October 3, 2011, protocol no. 04/2019 from March 5, 2019).

Additional information

Translated by I. Matiulko

Abbreviations: DNP, 2,4-dinitrophenol.

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Starinets, V.S., Lebedeva, E.V., Mikheeva, I.B. et al. Ultrastructural and Functional Changes in Liver Mitochondria in a Rat Model of Type I Diabetes Mellitus. BIOPHYSICS 64, 755–760 (2019). https://doi.org/10.1134/S0006350919050221

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  • DOI: https://doi.org/10.1134/S0006350919050221

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