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Preparation of Catalase Cross-Linked Aggregates Based on Vaterite Matrix

  • BIOLOGICALS TECHNOLOGY
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Abstract

A new technique has been developed for the synthesis of cross-linked catalase aggregates by treatment of the enzyme incorporated into the pores of vaterite microspheres with glutaraldehyde and subsequent dissolving of the inorganic matrix. The resulting aggregates have a spherical shape, a narrow particle size distribution, and a high specific activity. The number and enzyme activity of the cross-linked aggregates strongly depends on the rate of the matrix dissolution: mild conditions of dissolution made it possible to increase the number of formed protein particles, whose residual catalase specific activity was only 2 times less than that of the native enzyme. The storage stability of the cross-linked aggregates is comparable to that of the native enzyme of the same concentration.

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ACKNOWLEDGMENTS

The authors are grateful to the staff of Moscow State University A.N. Prusov for help in obtaining TEM images and A.A. Tatarintseva for assistance in obtaining SEM images.

Funding

This work was financially supported by the State Registration Topic AAAA-A21-121011290089-4. The equipment was purchased within the framework of the Moscow State University Development Program.

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Authors and Affiliations

  1. Department of Chemistry, Moscow State University, 119991, Moscow, Russia

    M. A. Tagirova, N. L. Eremeev, N. G. Balabushevich, D. V. Volodkin & N. L. Klyachko

  2. Research Institute “Nanotechnology and Nanomaterials,” Derzhavin Tambov State University, 392000, Tambov, Russia

    N. L. Klyachko

Authors
  1. M. A. Tagirova
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  2. N. L. Eremeev
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  3. N. G. Balabushevich
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  4. D. V. Volodkin
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  5. N. L. Klyachko
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Correspondence to N. L. Klyachko.

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The authors declare that they have no conflicts of interest.

This article does not contain any studies involving animals performed by any of the authors.

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Translated by I. Gordon

Abbreviations: Cat, catalase co-precipitated with vaterite after dissolution of the carbonate matrix; Cat–CLEAs, cross-linked catalase aggregates; CC, vaterite microspheres; Cat–GA, catalase co-precipitated with vaterite microspheres and treated with glutaraldehyde after dissolution of the carbonate matrix; CC–Cat, vaterite microspheres co-precipitated with catalase; CC–Cat–GA, vaterite microspheres co-precipitated with catalase and treated with glutaraldehyde; EDTA, ethylenediaminetetraacetic acid; GA, glutaraldehyde; SEM, scanning electron microscopy; TEM, transmission electron microscopy.

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Tagirova, M.A., Eremeev, N.L., Balabushevich, N.G. et al. Preparation of Catalase Cross-Linked Aggregates Based on Vaterite Matrix. Appl Biochem Microbiol 58, 923–931 (2022). https://doi.org/10.1134/S0003683822080075

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