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Effect of Transglutaminase on the Properties of Films Prepared from Chitosan and Gelatin

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Abstract

The production conditions were optimized, and the characteristics of biodegradable chitosan-gelatin films crosslinked with various microbial transglutaminases (transglutaminase 1, which contains maltodextrin, and transglutaminase 2, which contains maltodextrin, a milk protein) were studied. The film properties depended on both the cross-linking conditions and the type and ratio of components in the mixture. Gels and films prepared from chitosan and gelatin with transglutaminase 1 had very low characteristics as compared to films obtained with transglutaminase 2. Infrared spectroscopy, X-ray tomography, and scanning atomic force electron microscopy showed that transglutaminase 2 forms cross-links between both gelatin and chitosan amino groups, which improves the film characteristics. Films prepared from chitosan, gelatin, and glycerol crosslinked with transglutaminase 2 had the following characteristics: a strength of 15.8 MPa, a tensile strength of 220.0%, a thickness of 73.1 μm, and a density of 1.9 g/cm3. The biodegradability of the films depended on the composition and ranged from 20 to 30 days. The resulting films can serve as a matrix to obtain medical materials with antibacterial and wound-healing properties.

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REFERENCES

  1. Siracusa, V., Rocculi, P., Romani, S., and Rosa, M.D., Trends Food Sci. Technol., 2008, vol. 19, pp. 634–643.

    Article  CAS  Google Scholar 

  2. Kadimaliev, D.A., Telyatnik, V.I., Revin, V.V., Parshin, A.A., Allahverdi, S., Gunduz, G., Kezina, E.V., and Asik, N., BioResources, 2012, vol. 7, no. 2, pp. 1984–1993.

    Article  CAS  Google Scholar 

  3. Kadimaliev, D.A., Kezina, E.V., Telyatnik, V.I., Revin, V.V., Parchaykina, O.V., and Syusin, I.V., BioResources, 2015, vol. 10, no. 1, pp. 1644–1656.

    Article  Google Scholar 

  4. Revin, V.V., Kadimaliev, D.A., and Novokuptsev, N., BioResources, 2016, no. 2, pp. P. 3244–3258.

  5. Kadimaliev, D.A., Novokuptsev, N.V., Abd, A.J., Revin, V.V., Grunyushkin, I.P., and Pestov, N.A., J. Mater. Environ. Sci., 2018, vol. 9, no. 9, pp. 2539–2548.

    Google Scholar 

  6. RF Patent no. 2545293, 2015.

  7. Briassoulis, D., J. Polym. Environ., 2004, vol. 12, pp. 65–81.

    Article  CAS  Google Scholar 

  8. RF Patent no. 2604223, 2016.

  9. RF Patent no. 2720099, 2020.

  10. Kadimaliev, D.A., J. Biotechnol., 2015, vol. 208.

  11. Montgomery, R., Bioresour. Technol., 2004, vol. 91, pp. 1–29.

    Article  CAS  Google Scholar 

  12. Yang, L. and Paulson, A.T., Food Res. Int., vol. 33, pp. 563–570.

  13. RF Patent no. 2659175, 2018.

  14. Gromovykh, T.I., Sadykova, V.S., Lutsenko, S.V., Dmitrenok, A.S., Fel’dman, N.B., Danilchuk, T.N., and Kashirin, V.V., Appl. Biochem. Microbiol., 2017, vol. 53, no. 1, pp. 60–67.

    Article  CAS  Google Scholar 

  15. Skryabin, K.G., Vikhoreva, G.A., and Varlamov, V.P., Khitin i khitozan. Poluchenie, svoistva i primenenie (Chitin and Chitosan: Obtaining, Properties, and Application), Skryabin, K.G., Vikhorev, G.A., and Varlamov, V.P., Eds., Moscow: Nauka, 2002.

    Google Scholar 

  16. Vera, A., Tapia, C., and Abugoch, L., Int. J. Biol. Macromol., 2020, vol. 144, pp. 536–543.

    Article  CAS  Google Scholar 

  17. Minh, N.P., Nhan, N.P.T., Phuong, N.T.T., Vinh, T.Q., Van Quy, T., and Binh, L.T., J. Pharm. Sci. Res., 2019, vol. 11, no. 4, pp. 1487–1492.

    CAS  Google Scholar 

  18. Fernandez, J.G., Seetharam, S., Ding, C., Feliz, J., Doherty, E., and Ingber, D.E., Tissue Eng., 2017, vol. 23, nos. 3–4, pp. 135–142.

    Article  CAS  Google Scholar 

  19. RF Patent no. 2116314, 1997.

  20. Bykova, V.M. and Nemtsev, S.V., in Khitin i khitozan. Poluchenie, svoistva i primenenie (Chitin and Chitosan: Obtaining, Properties, and Application), Moscow: Nauka, 2002, pp. 7–23.

  21. Arzamastsev, O.S., Artemenko, S.E., Abdulin, V.F., and Arzamastsev, S.V., Vestn. SGTU, 2011, no. 4 (60), issue 2, pp. 112–114.

  22. Bradford, M.M., Anal. Biochem., 1976, vol. 72, pp. 248–254.

    Article  CAS  Google Scholar 

  23. ASTM D882-12. Standard Test Method for Tensile Properties of Thin Plastic Sheeting, West Conshohocken, PA: ASTM Int., 2012.

  24. Gartman, O.R. and Vorob’eva, V.M., Fundam. Issled., 2013, no. 3, pp. 1188–1192.

  25. Kuchina, Yu.A., Dolgopyatova, N.V., Novikov, V.Yu., Sagaidachnyi, V.A., and Morozov, N.N., Vestn. Mosk. Gos. Tech. Univ., 2012, vol. 15, no. 1, pp. 107–113.

    Google Scholar 

  26. Lawrie, G., Keen, I., Drew, B., Chandler-Temple, A., Rintoul, L., Fredericks, P., and Grondahl, L., Biomacromolecules, 2007, vol. 8, pp. 2533–2541.

    Article  CAS  Google Scholar 

  27. Rodrfguez-Nogales, J.M., Process Biochem., 2006, vol. 41, pp. 430–437.

    Article  Google Scholar 

  28. Minh, N.P., Nhan, N.P.T., Phuong, N.T.T., Vinh, T.Q., and Van Quy, T., J. Pharm. Sci. Res., 2019, vol. 11, no. 4, pp. 1487–1492.

    CAS  Google Scholar 

  29. Fernandez, J.G., Seetharam, S., Ding, C., Feliz, J., Doherty, E., and Ingber, D.E., Tissue Eng., 2017, vol. 23, nos. 3-4, pp. 135–142.

    Article  CAS  Google Scholar 

  30. Benjakul, S., Visessanguan, W., Phatchrat, S., and Tanaka, M., J. Food Biochem., 2003, vol. 27, pp. 53–66.

    Article  CAS  Google Scholar 

  31. Estaca, J., Gomez-Gullen, M.C., Fernandez-Martin, F., and Montero, P., Food Hydrocolloids, 2011, vol. 25, no. 6, pp. 1461–1469.

    Article  Google Scholar 

  32. Yampolskaya, G.P., Tarasevich, B.N., and Elenskiy, A.A., Kolloid. Zh., 2005, vol. 67, no. 3, pp. 426–432.

    Google Scholar 

  33. Jun-Hyun, O., Fish Aquat. Sci., 2012, vol. 15, no. 1, pp. 9–14.

    Google Scholar 

  34. Xu, Y.X., Kim, K.M., Hanna, M.A., and Nag, D., Ind. Crops Prod., 2005, vol. 21, pp. 185–192.

    Article  CAS  Google Scholar 

  35. Meenakshi, P., Noorjahan, S.E., Rajini, R., Venkateswarlu, U., Rose, C., and Sastry, T.P., Bull. Mater. Sci., 2002, vol. 25, pp. 25–29.

    Article  CAS  Google Scholar 

  36. Maklakova, A.A., Voron’ko, N.G., Derkach, S.R., Kadyrova, G.I., and Zotova, K.V., Vestn. Mosk. Gos. Tech. Univ., 2014, vol. 17, no. 1, pp. 53–60.

    Google Scholar 

  37. Derkach, S.R., Voron’ko, N.G., Maklakova, A.A., and Kondratyuk, Yu.V., Kolloid. Zh., 2014, vol. 76, no. 2, pp. 164–170.

    Google Scholar 

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Funding

This work was supported by the Ministry of Science and Higher Education of the Russian Federation (fulfillment of a state assignment) within the framework of scientific project no. FZRS-2020-0003.

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Authors and Affiliations

  1. Ogarev Mordovia State National Research University, Faculty of Biotechnology and Biology, 430005, Saransk, Russia

    D. A. Kadimaliev, O. V. Parchaykina, I. V. Syusin, A. N. Malafeev, A. A. Devyatkin & V. V. Revin

  2. Sechenov First Moscow State Medical University, Ministry of Health of the Russian Federation (Sechenov University), 119991, Moscow, Russia

    I. V. Chairkin

Authors
  1. D. A. Kadimaliev
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  2. O. V. Parchaykina
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  3. I. V. Syusin
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  4. I. V. Chairkin
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  5. A. N. Malafeev
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  6. A. A. Devyatkin
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  7. V. V. Revin
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Correspondence to D. A. Kadimaliev.

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The authors declare that they have no conflict of interest. This article does not contain any research involving humans or animals as research objects.

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Translated by P. Kuchina

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Kadimaliev, D.A., Parchaykina, O.V., Syusin, I.V. et al. Effect of Transglutaminase on the Properties of Films Prepared from Chitosan and Gelatin. Appl Biochem Microbiol 57, 366–372 (2021). https://doi.org/10.1134/S0003683821030042

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