Abstract
Rabbit and chicken (IgY) polyclonal antibodies were compared with respect to their performance in competitive enzyme linked immunoassays (ELISA) for the determination of opioid peptide β-casomorphin 7 (BCM-7) released from variant A1 of bovine β-casein. To obtain antibodies, the animals (four rabbits and four hens) were immunized (with similar regimes) with BCM-7 conjugated to bovine serum albumin. Comparison of the binding curves of biotinylated BCM-7 obtained with affinity-purified mammalian and avian antibodies immobilized on the surface of polystyrene microtiter immunoplates via passive adsorption (in optimal conditions) showed that rabbit antibodies captured biotinylated antigen 100 times more efficiently than hen antibodies within the given antibody panel. The most efficient rabbit antibodies were used to construct a highly sensitive, competitive ELISA for the detection of BCM-7 (the minimal detection limit was 0.2 ng/mL). The chicken antibodies proved unsuitable for such use due to their low affinity. These results indicate that it is necessary to make comparisons with methods based on mammalian antibodies in the construction of quantitative ELISA based on chicken polyclonal antibodies.
Similar content being viewed by others
REFERENCES
Schade, R., Calzado, E.G., Sarmiento, R., Chacana, P.A., Porankiewicz-Asplund, J., and Terzolo, H.R., Altern. Lab. Anim., 2005, vol. 33, no. 2, pp. 129–154.
Tambourgi, D.V., Vet. Immunol. Immunopathol., 2010, vol. 135, nos. 3–4, pp. 173–180.
Spillner, E., Braren, I., Greunke, K., Seismann, H., Blank, S., and Plessis, D., Biologicals, 2012, vol. 40, no. 5, pp. 313–322.
Lee, W., Syed, AtifA., Tan, S.C., and Leow, C.H., J. Immunol. Methods, 2017, vol. 447, pp. 71–85.
Júnior, F.Á., dos Pacheco, S.J., de Oliveira, S.I., Alves, M.A., Leonel, E.G., and Rodrigues, R.I., Ciencia Rural, 2018, vol. 48, no. 8. https://doi.org/10.1590/0103-8478cr20180250
Łupicka-Słowik, A., Walczak, M., Grzywa, R., Bobrek, K., Łęcka, M., Boivin, S., Gaweł, A., Stefaniak, T., Oleksyszyn, J., and Sieńczyk, M., Bioanalysis, vol. 6, no. 23, pp. 3197–3213.
Xiao, Y., Gao, X., Gannot, G., Emmert-Buck, M.R., Srivastava, S., Wagner, P.D., Amos, M.D., and Barke-r, P.E., Int. J. Cancer, 2008, vol. 122, no. 10, pp. 2178–2186.
Kuronen, I., Kokko, H., Mononen, I., and Parviainen, M., Eur. J. Clin. Chem. Clin. Biochem., 1997, vol. 35, no. 6, pp. 435– 440.
Di Lonardo, A., Marcante, M.L., Poggiali, F., Hamsoikova, E., and Venuti, A., Arch. Virol., 2001, vol. 146, no. 1, pp. 117–125.
de Paula, V.S., Silva AdosS., de Vasconcelos, G.A., Iff, E.T., Silva, M.E., Kappel, L.A., Cruz, P.B., and Pinto, M.A., J. Virol. Methods, 2011, vol. 171, no. 1, pp. 102–106.
Nafea, N.M., Sabbah, M.A., Al-Suhail, R., Mahdavi, A.H., and Asgary, S., Adv. Biomed. Res., 2015, vol. 4, p. 100. https://doi.org/10.4103/2277-9175.156678
Almeida, C.M.C., Quintana-Flores, V.M., Medina-Acosta, E., Schriefer, A., Barral-Netto, M., and Dias da Silva, W., Scand. J. Immunol., 2003, vol. 57, no. 6, pp. 573–582.
Chalghoumi, R., Théwis, A., Portetelle, D., and Beckers, Y., Poult. Sci., 2008, vol. 87, no. 1, pp. 32–40.
Meenatchisundaram, S., Shanmugam, V., and Anjali, V.M., J. Basic. Clin. Pharm., 2011, vol. 2, no. 2, pp. 109–114.
Ferreira Júnior, Á., Santiago, F.M, Silva, M.V, Ferreira, F.B, Macêdo Júnior, A.G, Mota, C.M, Faria, M.S, Silva Filho, H.H., Silva, D.A, Cunha-Júnior, J.P., Mineo, J.R., and Mineo, T.W., PLoS One, 2012, vol. 7, no. 7. e40391. https://doi.org/10.1371/journal.pone.0040391
Lei, J.H., Su, B.T., Xu, H., Shen, J.L., Guan, X.H., Feng, Z.Q., Li, Y.L., Xu, M.X., and Liu, W.Q., Am. J. Trop. Med. Hyg., 2011, vol. 85, no. 6, pp. 1054–1059.
Matsuda, H., Mitsuda, H., Nakamura, N., Furusawa, S., Mohri, S., and Kitamoto, T., FEMS Immunol. Med. M-icrobiol., 1999, vol. 23, no. 3, pp. 189–194.
Pechelyulko, A.A., Tarakanova, Yu.N., Dmitriev, D.A., Massino, Yu.S., Segal, O.L., Lavrov, V.F., and Dmitriev, A.D., Appl. Biochem. Microbiol., 2017, vol. 53, no. 1, pp. 114–122.
Kost, N.V., Sokolov, O.Y., Kurasova, O.B., Dmitriev, A.D., Tarakanova, J.N., Gabaeva, M.V., Zolotarev, Y.A., Dadayan, A.K., Grachev, S.A., Korneeva, E.V., Mikheeva, I.G., and Zozulya, A.A., Peptides, 2009, vol. 30, no. 10, pp. 1854–1860.
Pardue, H.L., Clin. Chem., 1997, vol. 43, no. 10, pp. 1831–1837.
Dmitriev, A.D., Tarakanova, J.N., Yakovleva, D.A., Dmitriev, D.A., Phartooshnaya, O.V., Kolyaskina, G.I., Massino, Y.S., Borisova, O.V., Segal, O.L., Smir-nova, M.B., Ulanova, T.I., and Lavrov, V.F., J. Immunoassay Immunochem., 2013, vol. 34, no. 4, pp. 414–437.
Tarakanova, Yu.N., Dmitriev, A.D., Massino, Yu.S., Pechelyul’ko, A.A., Segal, O.L., Skoblov, Yu.S., Ulanova, T.I., Lavrov, V.F., and Dmitriev, D.A., Appl. Biochem. Microbiol., 2015, vol. 51, no. 4, pp. 462–469.
Li, D., Cui, Y., Morisseau, C., Gee, S.J., Bever, C.S., Liu, X., Wu, J., Hammock, B.D., and Ying, Y., Anal. Chem., 2017, vol. 89, no. 11, pp. 6248–6256.
Butler, J.E., in Methods in Molecular Medicine: Molecular Diagnosis of Infectious Diseases, Decker, J. and Reischl, U., Eds., Totowa, N. J.: Humana Press Inc., 2004, pp. 333–372.
Küllenberg de Gaudry, D., Lohner, S., Schmucker, C., Kapp, P., Motschall, E., Hörrlein, S., Röger, C., and Meerpohl, J.J., Nutr. Rev., 2019, vol. 77, no. 5, pp. 278–306.
Yannakis, J. and Ozimek, L., Milchwissenschaft, 1998, vol. 53, no. 8, pp. 436–440.
Yannakis, J., Development of IgY antibodies in egg-yolk against beta-casomorphin-7, A Thesis Submitted to the Faculty of Graduate Studies and Research for the Degree of Master of Science, Canada, University of Alberta, 1997.
Kaufman, J., Trends Immunol., 2018, vol. 39, no. 5, pp. 367–379.
Derbinski, J., Pinto, S., Rosch, S., Hexel, K., and Kyewski, B., Proc. Natl. Acad. Sci. U. S. A., 2008, vol. 105, no. 2, pp. 657–662.
Enjapoori, A.K., Kukuljan, S., Dwyer, K.M., and Sharp, J.A., Nutrition, 2019, vol. 57, pp. 259–267.
Lonnerdal, B., Bergstrom, S., Andersson, Y., Hjalmarsson, K., Sundqvist, A.K., and Hernell, O., FEBS Lett., 1990, vol. 269, no. 1, pp. 153– 156.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of Interest. The authors declare that they have no conflict of interest.
Statement on Animal Welfare. All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.
Additional information
Translated by M. Novikova
Rights and permissions
About this article
Cite this article
Pechelyulko, A.A., Tarakanova, Y.N., Dmitriev, D.A. et al. Comparative Analysis of the Efficiency of Chicken and Rabbit Antibodies in Competitive Enzyme Linked Immunoassay for the Detection of Bovine Beta-Casomorphin 7. Appl Biochem Microbiol 55, 704–710 (2019). https://doi.org/10.1134/S0003683819060103
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S0003683819060103