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Purification and Characterization of a Novel (R)-1-Phenylethanol Dehydrogenase from Lysinibacillus sp. NUST506

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Abstract

A novel (R)-1-phenylethanol dehydrogenase was successfully purified from Lysinibacillus sp. NUST506 by preparative polyacrylamide gel electrophoresis. The enzyme is a NAD+-dependent oxidoreductase. The molecular weight of the (R)-1-phenylethanol dehydrogenase measured by SDS-PAGE was about 28 kDa. Furthermore, the optimal reaction conditions for the oxidative reaction were 70°C and pH 9.5 and for the reductive reaction were 65°C and pH 6.5. Under the optimal conditions, the KM and kcat values with (R)-1-phenylethanol as a substrate were found to be 0.78 mM and 123 s–1 and with acetophenone they were 0.56 mM and 125 s–1, respectively. The (R)-1-phenylethanol dehydrogenase became more stable at pH 9.5 in comparison with pH 5.0 and high stability was noticed at 4 and 37°C. Properties of the enzyme place it as a promising candidate for industrial applications.

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References

  1. Abate, A., Brenna, E., Costantini, A., Fuganti, C., Gatti, F.G., Malpezzi, L., and Serra, S., J. Org. Chem., 2006, vol. 71, no. 14, pp. 5228–5240.

    Article  CAS  PubMed  Google Scholar 

  2. Mandal, D., Ahmad, A., Islam, K.M., and Kumar, R., J. Mol. Catal. B: Enzym., 2004, vol. 27 nos. 2–3, pp. 61–63.

    Article  CAS  Google Scholar 

  3. Goswami, A. and Goswami, J., Tetrahedron Lett., 2005, vol. 46, no. 25, pp. 4411–4413.

    Article  CAS  Google Scholar 

  4. Suginaka, K., Hayashi, Y., and Yamamoto, Y., Tetrahedron: Asyrametry, 1996, vol. 27, no. 35, pp.1153–1158.

    Article  Google Scholar 

  5. Ivanov, A.E. and Schneider, M.P., J. Mol. Catal. B: Enzym., 1997, vol. 3, no. 6, pp. 303–309.

    Article  CAS  Google Scholar 

  6. Hoffmann, I., Silva, V.D., and Nascimento, M.D.G., J. Braz. Chem. Soc., 2011, vol. 22, no. 8, pp. 1559–1567.

    Article  CAS  Google Scholar 

  7. Faraldos, J.A., Giner, J., Smith, D.H., Wilson, M., Ronhovde, K., Wilson, E., et al., J. Chem. Educ., 2011, vol. 88, no. 3, pp. 334–336.

    Article  CAS  Google Scholar 

  8. Farbood, M.I., Blocker, R.W., Arvizzigno, J., and Muralidhara R., US Patent no. US 6602535 B1, 2003.

  9. Chua, L.S. and Sarmidi, M.R., J. Mol. Catal. B: Enzym., 2004, vol. 28, nos. 2–3, pp. 111–119.

    Article  CAS  Google Scholar 

  10. Yun, H., Yang, Y., Cho, B., Hwang, B., and Kim, B., Biotechnol. Lett., 2003, vol. 25, no. 10, pp. 809–814.

    Article  CAS  PubMed  Google Scholar 

  11. Homola, P., Kurák, T., Illeová, V., and Polakovic, M., Food Bioprod. Process., 2015, vol. 96, pp. 126–132.

    Article  CAS  Google Scholar 

  12. Rauter, M., Kasprzak, J., Denter, S., Becker, K., Baronian, K., Bode, R., et al., J. Mol. Catal. B: Enzym., 2014, vol. 108, no. 1, pp. 72–76.

    Article  CAS  Google Scholar 

  13. Rauter, M., Kasprzak, J., Becker, K., Baronian, K., Bode, R., Kunze, G., and Vorbrodt, H.M., J. Mol. Catal. B: Enzym., 2014, vol. 104, no. 2, pp. 8–16.

    Article  CAS  Google Scholar 

  14. Kasprzak, J., Bischoff, F., Rauter, M., Becker, K., Baronian, K., Bode, R., et al., J. Biochem. Eng., 2016, vol. 106, pp. 107–117.

    Article  CAS  Google Scholar 

  15. Li, Y.L., Xu, J.H., and Xu, Y., J. Mol. Catal. B: Enzym., 2010, vol. 64, nos. 1–2, pp. 48–52.

    Article  CAS  Google Scholar 

  16. Qian, W.K., Zhang, L., Sun, H.F., and Liu, J.H., Adv. Synth. Catal., 2012, vol. 354, no. 17, pp. 3231–3236.

    Article  CAS  Google Scholar 

  17. Hummel, W., Appl. Microbiol. Biotechnol., 1990, vol. 34, no. 1, pp. 15–19.

    Article  CAS  Google Scholar 

  18. Hummel, W., Adv. Biochem. Eng. Biotechnol., 1997, vol. 58, no. 4, pp. 145–184.

    CAS  PubMed  Google Scholar 

  19. Cripps, R.E. and Trudgill, P.W., Eur. J. Biochem., 1978, vol. 86, pp. 175–186.

    Article  CAS  PubMed  Google Scholar 

  20. Wang, M. and Yuan, W.Q., Bioresour. Technol., 2015, vol. 193, pp. 171–177.

    Article  CAS  PubMed  Google Scholar 

  21. Han, Y.J. and Chen, H.Z., J. Mol. Catal. B: Enzym., 2010, vol. 63, no. 3–4, pp. 135–140.

    Article  CAS  Google Scholar 

  22. Laemmli, U.K., Nature, 1970, vol. 227, no. 5259, pp. 680–685.

    Article  CAS  PubMed  Google Scholar 

  23. Zhou, S., Zhang S.C., Lai, D.Y., Zhang, S.L., and Chen, Z.M., Biotechnol. Lett., 2013, vol. 35, no. 3, pp. 359–365.

    Article  CAS  PubMed  Google Scholar 

  24. Yanai, H., Doi, K., and Ohshima, T., Appl. Environ. Microb., 2009, vol. 75, no. 6, pp. 1758–1763.

    Article  CAS  Google Scholar 

  25. Hirakawa, H., Kamiya, N., Kawarabayasgi, Y., and Nagamune, T., J. Biosci. Bioeng., 2004, vol. 97, no. 3, pp. 202–206.

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Bioengineering, Nanjing University of Science and Technology, Nanjing, 210094, People’s Republic of China

    X. Zhang, J. Yang, C. Yang, X. Chen, B. Bao, D. Li, R. Shi, J. Wang, S. Pu & X. Zhang

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  1. X. Zhang
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  2. J. Yang
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  3. C. Yang
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  4. X. Chen
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  5. B. Bao
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  6. D. Li
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  8. J. Wang
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Correspondence to D. Li.

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Zhang, X., Yang, J., Yang, C. et al. Purification and Characterization of a Novel (R)-1-Phenylethanol Dehydrogenase from Lysinibacillus sp. NUST506. Appl Biochem Microbiol 54, 149–154 (2018). https://doi.org/10.1134/S0003683818020126

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  • DOI: https://doi.org/10.1134/S0003683818020126

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