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Biosensors based on the luminous bacteria Photobaterium phosphoreum immobilized in polyvinyl alcohol cryogel for the monitoring of ecotoxicants

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Abstract

Immobilization of Photobacterium phosphoreum bacteria in polyvinyl alcohol cryogel was performed in order to develop biosensors used for ecotoxicant biomonitoring. The immobilization procedure, storage, and application of the immobilized cells for biomonitoring were optimized. It was shown that the immobilized cells demonstrate significantly higher stability and a longer duration of light emission than free bacteria. A discrete analysis of heavy metals and chlorophenols was conducted using the obtained biosensor samples.

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Authors and Affiliations

  1. Lomonosov Moscow State University, Moscow, 119991, Russia

    E. N. Efremenko, O. V. Senko, L. E. Aleskerova, K. A. Alenina, M. M. Mazhul & A. D. Ismailov

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  1. E. N. Efremenko
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  2. O. V. Senko
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  3. L. E. Aleskerova
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  4. K. A. Alenina
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  5. M. M. Mazhul
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  6. A. D. Ismailov
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Corresponding author

Correspondence to E. N. Efremenko.

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Original Russian Text © E.N. Efremenko, O.V. Senko, L.E. Aleskerova, K.A. Alenina, M.M. Mazhul, A.D. Ismailov, 2014, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2014, Vol. 50, No. 5, pp. 490–496.

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Efremenko, E.N., Senko, O.V., Aleskerova, L.E. et al. Biosensors based on the luminous bacteria Photobaterium phosphoreum immobilized in polyvinyl alcohol cryogel for the monitoring of ecotoxicants. Appl Biochem Microbiol 50, 477–482 (2014). https://doi.org/10.1134/S0003683814050032

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  • DOI: https://doi.org/10.1134/S0003683814050032

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