Original paper
Genetic Diversity among different Geographical Population of Rhyzopertha dominica in China as revealed by AFLP (Coleoptera: Bostrichidae
[Genetische Diversität unter verschiedenen geografischen Populationen von Rhyzopertha dominica in China, nachgewiesen durch AFLP (Coleoptera: Bostrichidae)]
Song, X; Akinkurolere, R; Wang, P; Zhang, H
Entomologia Generalis Volume 33 Number 1-2 (2011), p. 49 - 62
56 references
published: Jun 30, 2011
DOI: 10.1127/entom.gen/33/2011/49
ArtNo. ESP146003301003, Price: 15.40 €
Abstract
Rhyzopertha dominica (Fabricius 1792) is one of the major coleopterous pests of stored products in China and around the world. It causes serious losses to stored grains. In this study, the genetic diversity and structure of 13 populations (97 individuals) of R dominica were examined using Amplified Fragment Length Polymorphism (AFLP) analysis. Four primer combinations were selected to identify a total of 128 polymorphic loci ranging from 50 to 450 bp. The percentage of polymorphic loci (Pp) was 51.88 % at the population level and 84.87 % at the species level, respectively. Nei’s gene diversity (h) and Shannon’s Information index (I) had similar trendwith the percentage of polymorphism. Thus, the estimated genetic diversity using these three indicators revealed a high level of genetic variation within each R. dominica population. At the species level, Analysis of Molecular Variance (AMOVA) revealed a significantly greater genetic variation within populations (81.11 %) than among populations (18.89 %). The Mantel test of isolation by distance revealed that there was no significant correlation between Nei’s genetic distance and geographical distance (Lg) among all populations (r = 0.20787, P = 0.0519 >0.05). A Neighbour Joining dendrogram based on Nei’s unbiased genetic distance revealed four distinct groups that did not correspond to the geographical regions. This result suggests that isolation by distance does not describe the pattern of gene fow among R. dominica populations. How different ecology of grain storage area and depot type could influence R. dominica population genetic structure were also discussed.
Kurzfassung
Rhyzopertha dominica (Fabricius 1792) ist einer der wichtigsten Vorratsschädlinge in China und der ganzen Welt. Er verursacht schwere Verluste an gelagertem Getreide. In dieser Studie wurde die genetische Diversität und Struktur von 13 Populationen (97 Individuen) der Art R dominica mittels (Amplified Fragment Length Polymorphismus)AFLP-Analyse untersucht. Vier Primer-Kombinationen wurden ausgewählt, um insgesamt 128 polymorphe Loci im Bereich von 50 bis 450 bp zu identifzieren.
Keywords
Rhyzopertha dominica (Fabricius 1792) • AFLP • genetic diversity • genetic structure