Studying cellular mechanics using magnetic tweezers and two-photon microscopy

Hayden Huang; Chen-Yuan Dong; Hyuk-Sang Kwon; Jason D. B. Sutin; Roger D. Kamm; Peter T. C. So

doi:10.1117/12.426763

10 May 2001 Studying cellular mechanics using magnetic tweezers and two-photon microscopy

Hayden Huang, Chen-Yuan Dong, Hyuk-Sang Kwon, Jason D. B. Sutin, Roger D. Kamm, Peter T. C. So

Proceedings Volume 4260, Optical Diagnostics of Living Cells IV; (2001) https://doi.org/10.1117/12.426763
Event: BiOS 2001 The International Symposium on Biomedical Optics, 2001, San Jose, CA, United States

Abstract

The ability to apply quantifiable mechanical stresses at the microscopic scale is critical for studying cellular responses to mechanical forces. This necessitates the use of force transducers that can apply precisely controlled forces to cells while monitoring the responses non- invasively. This paper describes the development of a micro manipulation workstation integrating two-photon, 3-D imaging with a high-force, uniform-gradient, magnetic manipulator. The uniform-gradient magnetic field applies nearly equal forces to a large cell population, permitting statistical quantification of select molecular responses to mechanical stresses. The magnetic transducer design is capable of exerting over 200 pN of force on 4.5 micrometers diameter paramagnetic particles and over 800 pN on 5.0 micrometers ferromagnetic particles. These forces vary less than 10% over an area 200 x 200 micrometers ². The compatibility with the use of high numerical aperture (approximately equals 1.0) objectives is an integral part of the workstation design allowing sub- micron resolution 3-D two-photon imaging. Three dimensional maps of cellular deformation under localized mechanical strain are reported. These measurements indicate that the response of cells to large focal stresses is not always a local deformation.

Citation Download Citation

Hayden Huang, Chen-Yuan Dong, Hyuk-Sang Kwon, Jason D. B. Sutin, Roger D. Kamm, and Peter T. C. So "Studying cellular mechanics using magnetic tweezers and two-photon microscopy", Proc. SPIE 4260, Optical Diagnostics of Living Cells IV, (10 May 2001); https://doi.org/10.1117/12.426763

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