Nanoscale holographic tomography of heart tissue with x-ray waveguide optics

M. Reichardt; J. Frohn; M. Töpperwien; J.-D. Nicolas; A. Markus; F. Alves; T. Salditt

doi:10.1117/12.2276648

25 September 2017 Nanoscale holographic tomography of heart tissue with x-ray waveguide optics

M. Reichardt, J. Frohn, M. Töpperwien, J.-D. Nicolas, A. Markus, F. Alves, T. Salditt

Proceedings Volume 10391, Developments in X-Ray Tomography XI; 1039105 (2017) https://doi.org/10.1117/12.2276648
Event: SPIE Optical Engineering + Applications, 2017, San Diego, California, United States

Abstract

In this work we present x-ray phase-contrast tomography of heart tissue from mouse, combining computed tomography (CT) scans with laboratory and synchrotron radiation. The work serves as a proof-of-concept that the cyto-architecture and in particular the myofibril orientation can be assessed in three dimensions (3D) by phase-contrast CT. We demonstrate the synergistic use of laboratory μ-CT and of the high resolution synchrotron setup based on waveguide optics. Details on preparation, instrumentation and analysis are given, as a state of the art reference for heart tissue tomography, and as a starting point for further progress.

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Citation Download Citation

M. Reichardt, J. Frohn, M. Töpperwien, J.-D. Nicolas, A. Markus, F. Alves, and T. Salditt "Nanoscale holographic tomography of heart tissue with x-ray waveguide optics", Proc. SPIE 10391, Developments in X-Ray Tomography XI, 1039105 (25 September 2017); https://doi.org/10.1117/12.2276648

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