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Establishment and characterization of a novel cell line derived from a human small cell lung carcinoma that secretes parathyroid hormone, parathyroid hormone-related protein, and pro-opiomelanocortin

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Abstract

There are few case reports describing small cell lung carcinoma (SCLC), which secrete parathyroid hormone (PTH)-related protein (PTHrP) and result in hypercalcemia. We have established a novel cell line, derived from a 37-year-old woman with SCLC, which produced PTH, PTH-rP, and a part of proopiomelanocortin (POMC), and led to hypercalcemia. The cell line, named SS-1, was grown as floating cell clusters in DMEM/F12 medium supplemented with 10% fetal bovine serum and had a population doubling time of 72 h. The modal chromosome number was 47 (88%); marker chromosomes were not observed. The SS-1 cell line secreted not only PTHrP but also PTH, and both were decreased by CaCl2 administration. Decreasing the concentration of Ca++ in the growth medium stimulated the secretion of both PTHrP and PTH. The cell line had calcium sensing receptor (Cas-R). Since PTHrP and PTH secretion from the SS-1 cells was related to Ca++ concentration in the growth medium, the cell line might be useful for the study of PTH-rP and PTH regulation as well as for SCLC analysis. In addition, the cells secreted N terminal POMC, the precursor of adrenocorticotropic hormone, in response to stimulation with corticotropin releasing hormone. In summary, we established a novel cell line, SS-1 from SCLC, which produced PTHrP, PTH and N terminal POMC.

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Authors and Affiliations

  1. Division of Diabetes, Metabolism and Endocrinology, Toho University School of Medicine, 6-11-1, Omori-Nishi, Ota-Ku, 143-8541, Tokyo, Japan

    Mayumi Ishikawa, Masako Shimojo, Hajime Ueshiba, Kumiko Tsuboi & Gen Yoshino

  2. Division of Respiratory Medicine, Toho University School of Medicine, Japan

    Kazuhiro Kimura

  3. Department of Internal Medicine, Isuzu Hospital, Tokyo

    Kazuhiro Kimura

  4. Department of Anatomy, Jikei University School of Medicine, Japan

    Toshiaki Tachibana & Hisashi Hashimoto

  5. Department of Internal Medicine, Kanagawa Prefectural Shiomidai Hospital, Yokohama-shi, Japan

    Masako Shimojo

  6. Department of Pathology, Toho University School of Medicine, Japan

    Kazutoshi Shibuya

Authors
  1. Mayumi Ishikawa
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  2. Kazuhiro Kimura
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  3. Toshiaki Tachibana
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  4. Hisashi Hashimoto
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  5. Masako Shimojo
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  6. Hajime Ueshiba
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  7. Kumiko Tsuboi
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  8. Kazutoshi Shibuya
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  9. Gen Yoshino
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Correspondence to Mayumi Ishikawa.

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Ishikawa, M., Kimura, K., Tachibana, T. et al. Establishment and characterization of a novel cell line derived from a human small cell lung carcinoma that secretes parathyroid hormone, parathyroid hormone-related protein, and pro-opiomelanocortin. Hum Cell 23, 58–64 (2010). https://doi.org/10.1111/j.1749-0774.2010.00082.x

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  • DOI: https://doi.org/10.1111/j.1749-0774.2010.00082.x

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