Abstract
Aim:
To investigate the role of intercellular potassium in tachyplesin-induced HL-60 cells apoptosis.
Methods:
The concentration of intercellular potassium, cell volume and mitochondrial membrane potential were examined by flow cytometry.
Results:
The concentration of intercellular potassium reduced in a time-dependent manner in tachyplesin-treated HL-60 cells. In addition, the loss of mitochondrial membrane potential was tightly coupled with the shrinkage of cell volume. Different caspase inhibitors protected against DNA degradation but did not prevent the loss of HL-60 cell viability induced by tachyplesin. Ba2+, which was a kind of blocker of volume-regulatory K+ channels, increased the viability of tachyplesin-treated HL-60 cells and maintained mitochondrial membrane potential and cell volume.
Conclusion:
Efflux of K+ was an important reason for apoptosis in tachyplesin-treated HL-60 cells. Efflux of K+ affected the viability of tachyplesin-treated HL-60 cells independent of the process of caspase activation.
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Project supported by the Administration of Traditional Chinese Medicine of Guangdong province (1050047).
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Zhang, Ht., Wu, J., Zhang, Hf. et al. Efflux of potassium ion is an important reason of HL-60 cells apoptosis induced by tachyplesin. Acta Pharmacol Sin 27, 1367–1374 (2006). https://doi.org/10.1111/j.1745-7254.2006.00377.x
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DOI: https://doi.org/10.1111/j.1745-7254.2006.00377.x
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