Crystallization and preliminary X-ray crystallographic studies of human 2',3'-cyclic nucleotide 3'-phosphodiesterase

The catalytic fragment of human 2',3'-cyclic nucleotide 3'-phosphodiesterase (hCNP-CF) has been crystallized by the hanging-drop vapour-diffusion method using polyethylene glycol 300 as the precipitating agent. The crystals belong to the orthorhombic space group P2₁2₁2₁, with unit-cell parameters a = 44.39, b = 55.35, c = 78.76 Å. There is one molecule per asymmetric unit. The crystals diffract to at least 1.8 Å resolution using synchrotron radiation and are suitable for X-ray structure analysis at high resolution.