Crystallization and preliminary X-ray analysis of the EVH1 domain of Vesl-2b

Proteins of the Homer/Vesl family are enriched at excitatory synapses and selectively bind to a proline-rich consensus sequence in group 1 metabotropic glutamate receptors via a domain that shows a strong similarity to the Ena/VASP homology 1 (EVH1) domains. EVH1 domains play an important role in actin cytoskeleton dynamics. Crystals of the EVH1 domain of murine Vesl-2b were obtained that diffract X-rays to 2.4 Å resolution. They belong to space group C2, with unit-cell parameters a = 112.8, b = 69.9, c = 54.9 Å, β = 110.7°, consistent with three molecules per asymmetric unit and a solvent content of 53%.