Crystallization and preliminary X-ray analysis of pig E3, lipoamide dehydrogenase

Pig heart lipoamide dehydrogenase was crystallized by the hanging-drop vapor-diffusion method. X-ray diffraction patterns show that the hexagonal crystals have unit-cell dimensions of a = b = 359.3, c = 140.5 Å. The crystal structure has been preliminarily solved by the molecular-replacement method in the space group P6₃22. Three dimeric molecules have been found in the asymmetric unit, one of them located on the crystallographic twofold axis.