Crystallization and preliminary X-ray diffraction studies of the oxygenating subunit of 3,6-diketocamphane monooxygenase from Pseudomonas putida

The oxygenating constituent of the 3,6-diketocamphane monooxygenase isozyme from Pseudomonas putida NCIMB 10007 has been crystallized under two different conditions. Crystals were initially grown from polyethylene glycol (PEG) 8000 and sodium acetate using the vapour-phase diffusion method. The crystals were of orthorhombic P2₁2₁2₁ space group, with cell dimensions a = 55.8, b = 94.5 and c = 163.7 Å and diffracted to 2.8 Å resolution. More recently, improved crystals, which diffracted beyond 2 Å, have been grown from ammonium sulfate. These crystals also belong to the orthorhombic P2₁2₁2₁ space group, with cell dimensions of a = 54.6, b = 93.2 and c = 154.1 Å. A full native data set to 2.5 Å resolution has been collected from the ammonium sulfate grown crystals.