RCC1 Is a Nuclear Protein Required for Coupling Activation of cdc2 Kinase with DNA Synthesis and for Start of the Cell Cycle
This extract was created in the absence of an abstract.
Excerpt
Through cell fusion of interphase cells with mitotic cells the chromosomes of interphase cells are condensed and show premature chromosome condensation (PCC), which is unique to a phase of the cell cycle and depends on the degree of chromatin packing (Rao et al. 1976). Thus, in one sense, the entire cell cycle can be considered in terms of a cycle of chromosome packing and unpacking.
After completion of DNA synthesis, the maturation-promoting factor (MPF) cyclin B/p34cdc2 complex was activated as histone HI kinase, and the cells entered mitosis. To ensure dependency of mitosis on the completion of DNA synthesis, cells possess a regulatory system that recognizes DNA synthesis and blocks the initiation of mitosis during the S phase. Mutants independent of such a regulation are useful tools for analyzing cell cycle regulation. Since aberrations in a critical step of the cell cycle are likely to be lethal, we have isolated...
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↵* Present address: Fred Hutchinson Cancer Research Center, 1124 Columbia Street, Seattle, Washington 98104.
