In Vitro Systems for Genetic Recombination of the DNAs of Bacteriophage T7 and Yeast 2-micron Circle
This extract was created in the absence of an abstract.
Excerpt
In the hope of gaining mechanistic insights into genetic recombination, we have focused our efforts on the development of systems that carry out this process in vitro. Two such systems are general recombination of bacteriophage T7 DNA promoted by T7-infected cell extracts (Lee and Sadowski 1983) and the site-specific recombination of the yeast 2-micron plasmid, catalyzed by the FLP protein of this plasmid (Vetter et al. 1983).
In this presentation we discuss our findings on the role of DNA synthesis in in vitro recombination of T7 DNA and present a model summarizing evidence suggesting that general recombination of T7 DNA in vitro takes place by reciprocal exchanges of duplex DNA. Then we discuss our studies with the FLP protein of the 2-micron plasmid of yeast. This protein has been hyperproduced in yeast and in Escherichia coli, and crude extracts and purified preparations of FLP from both sources perform highly efficient...
