Genetic and Biochemical Approaches to Analyzing Transformation by Rous Sarcoma Virus
This extract was created in the absence of an abstract.
Excerpt
When Rous sarcoma virus (RSV) infects a susceptible cell, numerous cellular alterations occur, including changes in cell shape, plasminogen activator (PA) production, hexose transport, surface fibronectin, adhesiveness, and growth properties (for review, see Hanafusa 1977). The expression of the src gene is necessary for the establishment and maintenance of all of these changes (Martin 1971; Hanafusa 1977), and recently a 60K phosphoprotein (pp60src) has been shown to be coded by the src gene and to have an associated protein kinase activity (Brugge and Erikson 1977; Purchio et al. 1978; Collett and Erikson 1978; Erikson et al. 1978; Levinson et al. 1978).
Understanding of how the expression of the src gene brings about the numerous cellular alterations that constitute the phenotype of transformed fibroblasts is hindered by the complexity and interrelatedness of the alterations themselves, as well as by the superficiality of our knowledge concerning their molecular basis. In this paper...
