Mosquito Transposon-Mediated Transgenesis
- 1Department of Microbiology and Molecular Genetics, University of California, Irvine, California 92697-4500, USA
- 2Department of Molecular Biology and Biochemistry, University of California, Irvine, California 92697-3900, USA
- ↵3Correspondence: aajames{at}uci.edu
Abstract
Transposon-mediated transgenesis of mosquito vectors of disease pathogens followed the early success of transgenesis in the vinegar fly, Drosophila melanogaster. The P transposable element used in Drosophila does not function canonically in mosquitoes, and repeatable, routine transgenesis in mosquitoes was not accomplished until new transposable elements were discovered and validated. A number of distinct transposons were subsequently identified that mediate the introduction of exogenous DNA in a stable and heritable manner in mosquito species, including members of the genera Aedes, Anopheles, and Culex. The most versatile element, piggyBac, is functional in all of these mosquito genera, as well as in many other insects in diverse orders, and has been used extensively outside the class. Transposon-mediated transgenesis of recessive and dominant marker genes and reporter systems has been used to define functional fragments of gene control sequences, introduce exogenous DNA encoding products beneficial to medical interests, and act as “enhancer traps” to identify endogenous genes with specific expression characteristics.
Footnotes
-
From the Mosquitoes collection, edited by Laura B. Duvall and Benjamin J. Matthews.
