Topic Introduction

Purification and Culture of Dorsal Root Ganglion Neurons

  1. J. Bradley Zuchero1
  1. Department of Neurobiology, School of Medicine, Stanford University, Stanford, California 94305

    Abstract

    Dorsal root ganglion neurons (DRGs) are sensory neurons that reside in ganglions on the dorsal root of the spinal cord. Here we introduce a method for the acute, prospective purification and culture of DRGs from rodents in a serum-free, defined medium, in the absence of glial cells. This immunopanning-based method facilitates the study of DRG biology and function.

    Footnotes

    • 1 Correspondence: brad.zuchero{at}gmail.com

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