In Situ Hybridization of Sectioned Honeybee (Apis mellifera) Tissues
- Laboratory for Evolution and Development, National Research Centre for Growth and Development, Biochemistry Department, University of Otago, Dunedin 9054, New Zealand
- ↵1Corresponding author (peter.dearden{at}otago.ac.nz)
INTRODUCTION
This protocol outlines how to carry out section in situ hybridization on honeybee larvae to detect expression of a gene of interest in individual cell types. It can also be modified to investigate gene expression in a sectioned honeybee brain. A digoxigenin-labeled probe is hybridized to fixed sections and the probe is detected by 4-nitro blue tetrazolium chloride (NBT)-5-bromo-4-chloro-3-indolyl-phosphate (BCIP) color development.
