Alcian Blue/Alizarin Red Staining of Cartilage and Bone of Short-Tailed Fruit Bat (Carollia perspicillata)

John J. Rasweiler; Chris J. Cretekos; Richard R. Behringer

doi:10.1101/pdb.prot5166

Alcian Blue/Alizarin Red Staining of Cartilage and Bone of Short-Tailed Fruit Bat (Carollia perspicillata)

¹ Department of Obstetrics and Gynecology, State University of New York Downstate Medical Center, Brooklyn, NY 11203, USA
² Department of Biological Sciences, Idaho State University, Pocatello, ID 83209, USA
³ Department of Molecular Genetics, University of Texas M.D. Anderson Cancer Center, Houston, TX 77030, USA

↵⁴Corresponding author (rrb{at}mdanderson.org)

This article is also available in Emerging Model Organisms: A Laboratory Manual, Vol. 1. CSHL Press, Cold Spring Harbor, NY, USA, 2009.

INTRODUCTION

This protocol is used to stain embryo skeleton with alcian blue and alizarin red at later stages of development, when there is significant replacement of the cartilaginous early skeleton with ossified bone. It has been used with good results on the short-tailed fruit bat Carollia perspicillata and other bat species from CS 20 through neonatal stages.