High-Throughput Paraffin Section In Situ Hybridization and Dual Immunohistochemistry on Mouse Tissues
- 1 Institute for Molecular Bioscience, University of Queensland, St. Lucia, 4072, Australia
- 2 Australian Stem Cell Centre, University of Queensland, St. Lucia, 4072, Australia
- ↵3Corresponding author (Melissa.Little{at}stemcellcentre.edu.au)
INTRODUCTION
Section in situ hybridization (SISH) is a high-resolution tool used to analyze gene expression patterns. This protocol utilizes the Tecan Freedom EVO150 platform to perform high-throughput SISH on paraffin sections to detect mRNA with a digoxigenin (DIG)-labeled probe. The slide is mounted and imaged before performing immunohistochemistry (IHC) on the same section. The dual reaction enables a marker of protein expression to be localized on the same section as the mRNA and facilitates more accurate annotation of the gene expression.
