In Vivo Electroporation of Neurons
This protocol was adapted from “A Practical Guide: In Vivo Electroporation of Neurons,” Chapter 12, in Imaging in Neuroscience and Development (eds. Yuste and Konnerth). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2005.INTRODUCTION
This protocol describes the electroporation of DNA constructs to drive in vivo gene expression in neurons during early chick development. Electroporation is a method of physically introducing DNA constructs into cells through the application of an electric field. This simple method is important as it allows the ectopic expression of transgenes with relative ease in most neurons.
