Protocol

Whole-Mount Immunocytochemistry in Xenopus

  1. Michael W. Klymkowsky1
  1. Molecular, Cellular, and Developmental Biology, University of Colorado Boulder, Boulder, Colorado 80309
  1. ↵1Correspondence: klym{at}colorado.edu

Abstract

To visualize the effects of experimental perturbations on normal cellular behavior, morphology, and intracellular organization, we use a simple whole-mount immunocytochemical method with Xenopus oocytes, explants, or embryos. This method is applicable to a wide range of systems, including human-induced pluripotent stem cell-derived organoids, and can be used with both chromogenic (horseradish peroxidase/diaminobenzidine) and fluorescent imaging.

Footnotes

  • From the Xenopus collection, edited by Hazel L. Sive.

| Table of Contents

This Article

  1. Published in Advance November 30, 2017, doi: 10.1101/pdb.prot097295 Cold Spring Harb Protoc 2018: pdb.prot097295- © 2018 Cold Spring Harbor Laboratory Press
  1. » Abstract
  2. Full Text
  3. Full Text (PDF)
  4. All Versions of this Article:
    1. pdb.prot097295v1
    2. 2018/1/pdb.prot097295 most recent

Article Category

  1. Protocol

Personal Folder

  1. Save to Personal Folders

Updates/Comments

  1. Submit Updates/Comments
  2. No Updates/Comments published
  3. Alert me when Updates/Comments are published

Share

Current Issue

  1. May 2024, 2024 (5)
  1. Current Issue