Protocol

Analysis of Cell Viability by the alamarBlue Assay

  1. Pradeep D. Uchil

Abstract

This protocol describes viability measurements for cell cultures in a 96-well tissue culture plate using alamarBlue (resazurin). The assay can be modified to accommodate larger plates; however, for a preliminary analysis of transfection reagents and parameters of the transfection protocol on cell viability, a 96-well plate format is the most cost effective.

Footnotes

  • From the Molecular Cloning collection, edited by Michael R. Green and Joseph Sambrook.

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  1. doi:10.1101/pdb.prot095489 Cold Spring Harb Protoc 2018: pdb.prot095489- © 2018 Cold Spring Harbor Laboratory Press
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