Protocol

Preparation of Yeast DNA Sequencing Libraries

  1. Gavin Sherlock1
  1. Department of Genetics, Stanford University Medical School, Stanford, California 94305-5120

    Abstract

    This protocol provides a detailed description of how to prepare a DNA sequencing library from yeast genomic DNA for use with the Illumina sequencing platform. This method does not require purchase of Illumina kits for library preparation but instead employs specific reagents purchased largely from New England BioLabs, which significantly reduces the cost of library preparation. Although we assume here that users intend to generate libraries with ∼400-bp insert sizes for paired-end sequencing, it is relatively straightforward to modify the shearing and size selection steps for longer or shorter inserts.

    Footnotes

    • 1 Correspondence: gsherloc{at}stanford.edu

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