Culturing Hybridoma Cell Lines for Monoclonal Antibody Production
Abstract
This protocol describes how to culture hybridoma cell lines (e.g., Thy1.1) for monoclonal antibody production. Supernatants harvested from such cultures can be used to purify various rodent neural cell types by immunopanning.
Footnotes
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↵1 Correspondence: jtw{at}stanford.edu
- © 2013 Cold Spring Harbor Laboratory Press