Protocol

Culturing Hybridoma Cell Lines for Monoclonal Antibody Production

  1. Jack T. Wang1
  1. Department of Neurobiology, Stanford University School of Medicine, Stanford, California 94305

    Abstract

    This protocol describes how to culture hybridoma cell lines (e.g., Thy1.1) for monoclonal antibody production. Supernatants harvested from such cultures can be used to purify various rodent neural cell types by immunopanning.

    Footnotes

    • 1 Correspondence: jtw{at}stanford.edu

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