A First-Generation Whole Genome–Radiation Hybrid Map Spanning the Mouse Genome

  1. Linda C. McCarthy1,5,
  2. Jonathan Terrett2,
  3. Maria E. Davis1,
  4. Catherine J. Knights1,
  5. Angela L. Smith1,
  6. Ricky Critcher1,
  7. Karin Schmitt3,
  8. Jim Hudson4,
  9. Nigel K. Spurr2, and
  10. Peter N. Goodfellow1,2
  1. 1Genetics Department, University of Cambridge, Downing Site, Cambridge CB2 3EH, UK; 2SmithKline Beecham Pharmaceuticals, New Frontiers Science Park, Harlow, Essex CM19 5AW, UK; 3Millenium Pharmaceuticals, Cambridge, Massachusetts 02139; 4Research Genetics, Inc., Huntsville, Alabama 35801

Abstract

We have assembled a first-generation anchor map of the mouse genome using a panel of 94 whole-genome–radiation hybrids (WG–RHs) and 271 sequence-tagged sites (STSs). This is the first genome-wide RH anchor map of a model organism. All of the STSs have been previously localized on the genetic map and are located 8.8 Mb apart on average. This mouse WG–RH panel, known as T31, has an average retention frequency of 27.6% and an estimated potential resolution of 145 kb, making it a powerful resource for efficient large-scale expressed sequence tag mapping.

[All of the mapping data for the maps presented here have been deposited at the Research Genetics, Inc., web site and can be freely accessed and downloaded athttp://www.resgen.com/.]

Footnotes

  • ↵5 Corresponding author.

  • E-MAIL lmc{at}mole.bio.cam.ac.uk; FAX 01223 333992.

    • Received August 4, 1997.
    • Accepted October 24, 1997.
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  1. doi: 10.1101/gr.7.12.1153 Genome Res. 7: 1153-1161 Cold Spring Harbor Laboratory Press

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