Xenopus Paraxial Protocadherin regulates morphogenesis by antagonizing Sprouty

Yingqun Wang; Patricia Janicki; Isabelle Köster; Corinna D. Berger; Christian Wenzl; Jörg Großhans; Herbert Steinbeisser

doi:10.1101/gad.452908

Xenopus Paraxial Protocadherin regulates morphogenesis by antagonizing Sprouty

¹ Institute of Human Genetics, University Heidelberg, 69120 Heidelberg, Germany;
² Center for Molecular Biology (ZMBH), University Heidelberg, 69120 Heidelberg, Germany

Abstract

Xenopus Paraxial Protocadherin (xPAPC) has signaling functions that are essential for convergent extension (CE) movements and tissue separation during gastrulation. PAPC modulates components of the planar cell polarity (PCP) pathway, but it is not clear how PAPC is connected to β-catenin-independent Wnt-signaling. By yeast two-hybrid screen, we found that the intracellular domain of PAPC interacts with Sprouty (Spry), an inhibitor of CE movements. Upon binding to PAPC, Spry function is inhibited and PCP signaling is enhanced. Our data indicate that PAPC promotes gastrulation movements by sequestration of Spry and reveal a novel mechanism by which protocadherins modulate β-catenin-independent Wnt-signaling.

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Footnotes

↵3 Corresponding authors.

↵3 E-MAIL Herbert.Steinbeisser{at}med.uni-heidelberg.de; FAX 6221-565153
↵4 E-MAIL Yingqun.Wang{at}med.uni-heidelberg.de; FAX 6221-568884.
Supplemental material is available at http://www.genesdev.org.
Article is online at http://www.genesdev.org/cgi/doi/10.1101/gad.452908.
- Received August 14, 2007.
- Accepted January 18, 2008.