The Ino80 complex prevents invasion of euchromatin into silent chromatin
- Yong Xue1,3,
- Christopher Van2,3,
- Suman K. Pradhan1,
- Trent Su1,
- Jason Gehrke1,
- Benjamin G. Kuryan1,
- Tasuku Kitada1,
- Ajay Vashisht1,
- Nancy Tran1,
- James Wohlschlegel1,
- Craig L. Peterson2,4,
- Siavash K. Kurdistani1,4 and
- Michael F. Carey1,4
- 1Department of Biological Chemistry, David Geffen School of Medicine at University of California at Los Angeles, Los Angeles, California 90095, USA;
- 2Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
- Corresponding author: mcarey{at}mednet.ucla.edu
Abstract
Here we show that the Ino80 chromatin remodeling complex (Ino80C) directly prevents euchromatin from invading transcriptionally silent chromatin within intergenic regions and at the border of euchromatin and heterochromatin. Deletion of Ino80C subunits leads to increased H3K79 methylation and noncoding RNA polymerase II (Pol II) transcription centered at the Ino80C-binding sites. The effect of Ino80C is direct, as it blocks H3K79 methylation by Dot1 in vitro. Heterochromatin stimulates the binding of Ino80C in vitro and in vivo. Our data reveal that Ino80C serves as a general silencing complex that restricts transcription to gene units in euchromatin.
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Footnotes
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Supplemental material is available for this article.
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Article is online at http://www.genesdev.org/cgi/doi/10.1101/gad.256255.114.
- Received November 20, 2014.
- Accepted January 16, 2015.
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