Adenoviral DNA Replication: DNA Sequences and Enzymes Required for Initiation In vitro
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Excerpt
The development of cell-free systems for the replication of DNA has enabled diverse mechanisms for the priming of DNA synthesis at replication origins to be determined. Such varied mechanisms are discussed in this volume, including the self-priming of replication by the template DNA, as for the autonomous parvoviruses (Astell et al., this volume) and the priming by an RNA synthesized either by primase (Stayton et al.; Brown et al.; both this volume) or by RNA polymerase (Fuller et al., this volume). The development of a cell-free system for the replication of adenoviral DNA (Challberg and Kelly 1979) has paved the way for a detailed examination of a novel mechanism for the initiation of DNA synthesis, namely, priming by a deoxynucleotide that is covalently attached to a protein. Several aspects of this mechanism are discussed in this paper and by Lichy et al. (this volume).
A protein of 55,000 daltons (55K),...