ABSTRACT
Background Vasculopathy associated with diabetic neuropathy are important risk factors for the diabetic foot ulcers development. Diabetic foot ulcers is severe complication that occur in about 15% of people with diabetes, being able require hospitalization and amputation in its treatment.
Objective Design in silico the molecular structure of micro-ribonucleic acid (miRNA) overexpressed in diabetic foot ulcers healing.
Method We performed a careful search of the nucleotide sequence of 8 miRNAs over-expressed in diabetic foot ulcers, designing in silico the molecular structure of following miRNAs: miRNA-146a, miRNA-155, miRNA-132, miRNA-191, miRNA-21, miRNA-203a, miRNA-203b, and miRNA-210. The nucleotides were taken from GenBank of National Center for Biotechnology Information genetic sequence database. The sequences acquired were aligned with the Clustal W multiple alignment algorithms. The molecular modeling of structures was built using the RNAstructure, an automated miRNAs structure modelling server.
Results We showed a search for nucleotide sequence and the design of the molecular structure of following miRNA over-expressed in diabetic foot ulcers healing: miRNA-146a, miRNA-155, miRNA-132, miRNA-191, miRNA-21, miRNA-203a, miRNA-203b, and miRNA-210. We produced a tutorial on a molecular model of the 8 miRNAs overexpressed in the diabetic foot by processing in silico projection of their molecular structures.
Conclusion We show in silico secondary structures design of selected of 8 miRNAs over-expressed in diabetic foot ulcers healing by means of computational biology.
Competing Interest Statement
The authors have declared no competing interest.