Abstract
Premise of the study The family Salicaceae has proved taxonomically challenging, especially in the genus Salix, which is speciose and features frequent hybridization and polyploidy. Past efforts to reconstruct the phylogeny with molecular barcodes have failed to resolve the species relationships of many sections of the genus.
Methods We used the wealth of sequence data in the family to design sequence capture probes to target regions of 300-1200 base pairs of exonic regions of 972 genes.
Results We recovered sequence data for nearly all of the targeted genes in three species of Populus and three species of Salix. We present a species tree, discuss concordance among gene trees, as well as some population genomic summary statistics for these loci.
Conclusions Our sequence capture array has extremely high capture efficiency within the genera Populus and Salix, resulting in abundant phylogenetic information. Additionally, these loci show promise for population genomic studies.
Competing Interest Statement
The authors have declared no competing interest.
Footnotes
Email addresses: BJS: brian{at}biologicallyrelevant.com, SPD: spdifazio{at}mail.wvu.edu, QC: quentin.cronk{at}ubc.ca, TM: matao.yz{at}gmail.com, MSO: matt.olson{at}ttu.edu
Light revisions mostly pertaining to a Zenodo release of data associated with this paper, as well as completion of the SRA accession numbers for raw read data in Table S1