Krebs-Henseleit (KH) Buffer

118 mM NaCl

4.7 mM KCl

1.2 mM MgSO₄

1.25 mM CaCl₂

1.2 mM KH₂PO₄

25 mM NaHCO₃

11 mM glucose

Make one liter fresh on the day of isolation from 20x stocks (except the glucose). KH buffer has to be oxygenated by gassing the solution with 95% O₂/5% CO₂ until the pH is apporximately 7.4.

If difficulty is encountered with the quantity and/or quality of the isolated cells, which can be caused by variations in water quality, Joklik's media (GIBCO/BRL) can be used in place of calcium-free KH buffer.