From ORFeome to Biology: A Functional Genomics Pipeline

  1. Stefan Wiemann1,3,
  2. Dorit Arlt1,
  3. Wolfgang Huber1,
  4. Ruth Wellenreuther1,
  5. Simone Schleeger1,
  6. Alexander Mehrle1,
  7. Stephanie Bechtel1,
  8. Mamatha Sauermann1,
  9. Ulrike Korf1,
  10. Rainer Pepperkok2,
  11. Holger Sültmann1, and
  12. Annemarie Poustka1
  1. 1 Molecular Genome Analysis, German Cancer Research Center, 69120 Heidelberg, Germany
  2. 2 Cell Biology and Biophysics Programme, European Molecular Biology Laboratory, 69115 Heidelberg, Germany

Abstract

As several model genomes have been sequenced, the elucidation of protein function is the next challenge toward the understanding of biological processes in health and disease. We have generated a human ORFeome resource and established a functional genomics and proteomics analysis pipeline to address the major topics in the post-genome-sequencing era: the identification of human genes and splice forms, and the determination of protein localization, activity, and interaction. Combined with the understanding of when and where gene products are expressed in normal and diseased conditions, we create information that is essential for understanding the interplay of genes and proteins in the complex biological network. We have implemented bioinformatics tools and databases that are suitable to store, analyze, and integrate the different types of data from high-throughput experiments and to include further annotation that is based on external information. All information is presented in a Web database (http://www.dkfz.de/LIFEdb). It is exploited for the identification of disease-relevant genes and proteins for diagnosis and therapy.

Footnotes

  • Article and publication are at http://www.genome.org/cgi/doi/10.1101/gr.2576704.

  • 3 Corresponding author. E-MAIL s.wiemann{at}dkfz.de; FAX 49 6221 4252 4702.

    • Accepted May 12, 2004.
    • Received March 15, 2004.
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