Identification of Genes Encoding Amino Acid Permeases by Inactivation of Selected ORFs from the Synechocystis Genomic Sequence
Abstract
Genes encoding elements of four amino acid permeases were identified by insertional inactivation of ORFs from the genomic sequence of the cyanobacterium Synechocystis sp. strain PCC 6803 whose putative products are homologous to amino acid permease proteins from other bacteria. A transport system for neutral amino acids and histidine and a transport system for basic amino acids and glutamine were identified as ABC-type transporters, whereas Na+-dependent transport of glutamate was found to be mediated by at least two systems, the secondary permease GltS and a TRAP-type transporter. Except for GltS, substrate specificities of the identified permeases do not match those of previously characterized systems homologous to these permeases.
Footnotes
-
↵1 Corresponding author.
-
E-MAIL flores{at}cica.es; FAX 34 95 446 0065.
-
Article and publication are at http://www.genome.org/cgi/doi/10.1101/gr.196301.
-
- Received May 17, 2001.
- Accepted September 10, 2001.
- Cold Spring Harbor Laboratory Press
