1887

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Volume 89, Issue 10

Trypsin cleavage of the baculovirus occlusion-derived virus attachment protein P74 is prerequisite in infection Free

Abstract

Baculovirus occlusion-derived virions (ODVs) contain a number of infectivity factors essential for the initiation of infection in larval midgut cells. Deletion of any of these factors neutralizes infectivity by the route. We have observed that P74 of the group I alphabaculovirus multiple nucleopolyhedrovirus (AcMNPV) is N-terminally cleaved when a soluble form of the protein was incubated with insect midgut tissues under alkaline conditions and that cleavage was prevented by soybean trypsin inhibitor (SBTI). Presently, biological assays were carried out that suggest SBTI inhibits and trypsin enhances baculovirus infectivity. We developed a method to rescue infectivity of a P74 null virus involving co-transfection of viral DNA with a plasmid that transiently expresses . We used this plasmid rescue method to functionally characterize P74. A series of site-directed mutants were generated at the N terminus to evaluate if trypsin cleavage sites were necessary for function. Mutagenesis of R195, R196 and R199 compromised infectivity and rendered P74 resistant to midgut trypsin.

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2008-10-01
2024-04-19
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Trypsin cleavage of the baculovirus occlusion-derived virus attachment protein P74 is prerequisite in per os infection
J. Gen. Virol. 89, 2388 (2008); https://doi.org/10.1099/vir.0.2008/002543-0
/content/journal/jgv/10.1099/vir.0.2008/002543-0
/content/journal/jgv/10.1099/vir.0.2008/002543-0
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