1887

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Volume 94, Issue 2

Characterization of white spot syndrome virus immediate-early gene promoters Free

Abstract

Twenty-one immediate-early (IE) genes of white spot syndrome virus (WSSV) have been identified so far. However, the transcriptional regulation of WSSV IE genes remains largely unknown. In this report, the 5′ flanking regions of 18 WSSV IE genes were cloned and eight functional promoter regions were identified. WSSV IE gene promoters normally contained a TATA box approximately 30 bp upstream of the transcriptional initiation site. Also, the cyclic AMP response element (CRE; TGACGTCA) was frequently found within the WSSV IE promoter regions. Mutations of the CREs of WSSV IE promoters P403 and P465 reduced their activity significantly, suggesting that these elements have a role in WSSV IE gene transcription. Our findings provide a more global view of WSSV IE gene promoters and will facilitate the in-depth investigation of viral gene transcriptional regulation.

  • Received:
  • Accepted:
  • Published Online:
© 2013 SGM
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2013-02-01
2024-04-23
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References

  1. Butler J. E., Kadonaga J. T. 2002; The RNA polymerase II core promoter: a key component in the regulation of gene expression. Genes Dev 16:2583–2592 [View Article][PubMed]
     [Google Scholar]
  2. Chen A. J., Wang S., Zhao X. F., Yu X. Q., Wang J. X. 2011; Enzyme E2 from Chinese white shrimp inhibits replication of white spot syndrome virus and ubiquitinates its RING domain proteins. J Virol 85:8069–8079 [View Article][PubMed]
     [Google Scholar]
  3. Guarino L. A., Gonzalez M. A., Summers M. D. 1986; Complete sequence and enhancer function of the homologous DNA regions of Autographa californica nuclear polyhedrosis virus. J Virol 60:224–229[PubMed]
     [Google Scholar]
  4. Huang X. D., Zhao L., Zhang H. Q., Xu X. P., Jia X. T., Chen Y. H., Wang P. H., Weng S. P., Yu X. Q. other authors 2010; Shrimp NF-κB binds to the immediate-early gene ie1 promoter of white spot syndrome virus and upregulates its activity. Virology 406:176–180 [View Article][PubMed]
     [Google Scholar]
  5. Kemp C., Imler J. L. 2009; Antiviral immunity in drosophila. Curr Opin Immunol 21:3–9 [View Article][PubMed]
     [Google Scholar]
  6. Leu J. H., Yang F., Zhang X., Xu X., Kou G. H., Lo C. F. 2009; Whispovirus. Curr Top Microbiol Immunol 328:197–227 [View Article][PubMed]
     [Google Scholar]
  7. Li F., Li M., Ke W., Ji Y., Bian X., Yan X. 2009; Identification of the immediate-early genes of white spot syndrome virus. Virology 385:267–274 [View Article][PubMed]
     [Google Scholar]
  8. Lin F., Huang H., Xu L., Li F., Yang F. 2011; Identification of three immediate-early genes of white spot syndrome virus. Arch Virol 156:1611–1614 [View Article][PubMed]
     [Google Scholar]
  9. Liu W. J., Chang Y. S., Wang C. H., Kou G. H., Lo C. F. 2005; Microarray and RT-PCR screening for white spot syndrome virus immediate-early genes in cycloheximide-treated shrimp. Virology 334:327–341 [View Article][PubMed]
     [Google Scholar]
  10. Liu W. J., Chang Y. S., Wang A. H., Kou G. H., Lo C. F. 2007; White spot syndrome virus annexes a shrimp STAT to enhance expression of the immediate-early gene ie1 . J Virol 81:1461–1471 [View Article][PubMed]
     [Google Scholar]
  11. Liu W. J., Chang Y. S., Wang H. C., Leu J. H., Kou G. H., Lo C. F. 2008; Transactivation, dimerization, and DNA-binding activity of white spot syndrome virus immediate-early protein IE1. J Virol 82:11362–11373 [View Article][PubMed]
     [Google Scholar]
  12. Luo T., Li F., Lei K., Xu X. 2007; Genomic organization, promoter characterization and expression profiles of an antiviral gene PmAV from the shrimp Penaeus monodon . Mol Immunol 44:1516–1523 [View Article][PubMed]
     [Google Scholar]
  13. Manna P. R., Stocco D. M. 2007; Crosstalk of CREB and Fos/Jun on a single cis-element: transcriptional repression of the steroidogenic acute regulatory protein gene. J Mol Endocrinol 39:261–277 [View Article][PubMed]
     [Google Scholar]
  14. Marks H., Vorst O., van Houwelingen A. M., van Hulten M. C., Vlak J. M. 2005; Gene-expression profiling of White spot syndrome virus in vivo . J Gen Virol 86:2081–2100 [View Article][PubMed]
     [Google Scholar]
  15. Marks H., Ren X. Y., Sandbrink H., van Hulten M. C., Vlak J. M. 2006; In silico identification of putative promoter motifs of white spot syndrome virus. BMC Bioinformatics 7:309 [View Article][PubMed]
     [Google Scholar]
  16. Mayr B., Montminy M. 2001; Transcriptional regulation by the phosphorylation-dependent factor CREB. Nat Rev Mol Cell Biol 2:599–609 [View Article][PubMed]
     [Google Scholar]
  17. Murata T., Sato Y., Nakayama S., Kudoh A., Iwahori S., Isomura H., Tajima M., Hishiki T., Ohshima T. other authors 2009; TORC2, a coactivator of cAMP-response element-binding protein, promotes Epstein–Barr virus reactivation from latency through interaction with viral BZLF1 protein. J Biol Chem 284:8033–8041 [View Article][PubMed]
     [Google Scholar]
  18. Nupan B., Phongdara A., Saengsakda M., Leu J. H., Lo C. F. 2011; Shrimp Pm-fortilin inhibits the expression of early and late genes of white spot syndrome virus (WSSV) in an insect cell model. Dev Comp Immunol 35:469–475 [View Article][PubMed]
     [Google Scholar]
  19. Ochman H., Gerber A. S., Hartl D. L. 1988; Genetic applications of an inverse polymerase chain reaction. Genetics 120:621–623 [View Article][PubMed]
     [Google Scholar]
  20. Pearson M. N., Rohrmann G. F. 1995; Lymantria dispar nuclear polyhedrosis virus homologous regions: characterization of their ability to function as replication origins. J Virol 69:213–221[PubMed]
     [Google Scholar]
  21. Pedraza-Alva G., Zingg J. M., Jost J. P. 1994; AP-1 binds to a putative cAMP response element of the MyoD1 promoter and negatively modulates MyoD1 expression in dividing myoblasts. J Biol Chem 269:6978–6985[PubMed]
     [Google Scholar]
  22. Samten B., Townsend J. C., Weis S. E., Bhoumik A., Klucar P., Shams H., Barnes P. F. 2008; CREB, ATF, and AP-1 transcription factors regulate IFN-γ secretion by human T cells in response to mycobacterial antigen. J Immunol 181:2056–2064[PubMed] [CrossRef]
     [Google Scholar]
  23. Seth R. B., Sun L., Chen Z. J. 2006; Antiviral innate immunity pathways. Cell Res 16:141–147 [View Article][PubMed]
     [Google Scholar]
  24. Sharma-Walia N., George Paul A., Patel K., Chandran K., Ahmad W., Chandran B. 2010; NFAT and CREB regulate Kaposi’s sarcoma-associated herpesvirus-induced cyclooxygenase 2 (COX-2). J Virol 84:12733–12753 [View Article][PubMed]
     [Google Scholar]
  25. Smale S. T., Kadonaga J. T. 2003; The RNA polymerase II core promoter. Annu Rev Biochem 72:449–479 [View Article][PubMed]
     [Google Scholar]
  26. Wang Y., Kleespies R. G., Huger A. M., Jehle J. A. 2007; The genome of Gryllus bimaculatus nudivirus indicates an ancient diversification of baculovirus-related nonoccluded nudiviruses of insects. J Virol 81:5395–5406 [View Article][PubMed]
     [Google Scholar]
  27. Wang P. H., Gu Z. H., Wan D. H., Zhang M. Y., Weng S. P., Yu X. Q., He J. G. 2011; The shrimp NF-κB pathway is activated by white spot syndrome virus (WSSV) 449 to facilitate the expression of WSSV069 (ie1), WSSV303 and WSSV371 . PLoS ONE 6:e24773 [View Article][PubMed]
     [Google Scholar]
  28. Yang F., He J., Lin X., Li Q., Pan D., Zhang X., Xu X. 2001; Complete genome sequence of the shrimp white spot bacilliform virus. J Virol 75:11811–11820 [View Article][PubMed]
     [Google Scholar]
  29. Yuan Y. 2005; Identification and characterization of herpesviral immediate-early genes. Methods Mol Biol 292:231–244 [View Article]
     [Google Scholar]
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Characterization of white spot syndrome virus immediate-early gene promoters
J. Gen. Virol. 94, 387 (2013); https://doi.org/10.1099/vir.0.047274-0
/content/journal/jgv/10.1099/vir.0.047274-0
/content/journal/jgv/10.1099/vir.0.047274-0
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