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Volume 9, Issue 7

A hybrid and poly-polish workflow for the complete and accurate assembly of phage genomes: a case study of ten przondoviruses Open Access

Abstract

Bacteriophages (phages) within the genus are T7-like podoviruses belonging to the subfamily , within the family and have a highly conserved genome organisation. The genomes of these phages range from 37 to 42 kb in size, encode 50–60 genes and are characterised by the presence of direct terminal repeats (DTRs) flanking the linear chromosome. These DTRs are often deleted during short-read-only and hybrid assemblies. Moreover, long-read-only assemblies are often littered with sequencing and/or assembly errors and require additional curation. Here, we present the isolation and characterisation of ten novel przondoviruses targeting spp. We describe HYPPA, a brid and oly-polish hage ssembly workflow, which utilises long-read assemblies in combination with short-read sequencing to resolve phage DTRs and correcting errors, negating the need for laborious primer walking and Sanger sequencing validation. Our assembly workflow utilised Oxford Nanopore Technologies for long-read sequencing for its accessibility, making it the more relevant long-read sequencing technology at this time, and Illumina DNA Prep for short-read sequencing, representing the most commonly used technologies globally. Our data demonstrate the importance of careful curation of phage assemblies before publication, and prior to using them for comparative genomics.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): assembly , bacteriophage , Klebsiella , phage , Przondovirus and sequencing
Funding
This study was supported by the:
  • Biotechnology and Biological Sciences Research Council (Award BB/R012504/1)
    • Principle Award Recipient: MarkA. Webber
  • Wellcome Trust (Award 220876/Z/20/Z)
    • Principle Award Recipient: LindsayJ. Hall
  • Wellcome Trust (Award 100974/C/13/Z)
    • Principle Award Recipient: LindsayJ. Hall
  • Biotechnology and Biological Sciences Research Council (Award BB/CCG1860/1)
    • Principle Award Recipient: DavidJ. Baker
  • Biotechnology and Biological Sciences Research Council (Award BB/R012490/1)
    • Principle Award Recipient: EvelienM Adriaenssens
  • Medical Research Council (Award MR/R015937/1)
    • Principle Award Recipient: ClaireK. A. Elek
© 2023 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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2024-05-16
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A hybrid and poly-polish workflow for the complete and accurate assembly of phage genomes: a case study of ten przondoviruses
M Gen 9, 001065 (2023); https://doi.org/10.1099/mgen.0.001065
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