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Volume 6, Issue 12

Comparative genome analyses of reveals two potential novel subspecies Open Access

Abstract

is an emerging opportunistic pathogen implicated in nosocomial infections. Comparative genome analyses may provide better insights into its genomic structure, functions and evolution. The present analysis showed that has an open pan-genome. Approximately 36.8% of putative virulence genes were identified in the accessory regions of . Phylogenetic analyses revealed two potential novel subspecies of , supported by evidence from ANIb (average nucleotide identity using ) and GGDC (Genome to Genome Distance Calculator) analyses. We identified 74 genomic islands (GIs) in Subspecies 1 and 23 GIs in Subspecies 2. All Subspecies 2-harboured GIs were not found in Subspecies 1, indicating that they might have been acquired by Subspecies 2 after their divergence. Subspecies 2 has more defence genes than Subspecies 1, suggesting that it might be more resistant to the insertion of foreign DNA and probably explaining why Subspecies 2 has fewer GIs. Positive selection analysis suggest that has a lower selection pressure compared to non-pathogenic mycobacteria. Thirteen genes were positively selected and many were involved in virulence.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): bacterial defence genes , comparative genomics , dN/dS , genomic islands , M. abscessus , M. immunogenum , non-tuberculous mycobacteria , rapidly growing mycobacteria , subspecies , taxonomy and virulence
Funding
This study was supported by the:
  • Wenzhou-Kean University (Award 5000105)
    • Principle Award Recipient: SiewWoh Choo
© 2020 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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2020-12-09
2024-04-28
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Comparative genome analyses of Mycobacteroides immunogenum reveals two potential novel subspecies
M Gen 6, e000495 (2020); https://doi.org/10.1099/mgen.0.000495
/content/journal/mgen/10.1099/mgen.0.000495
/content/journal/mgen/10.1099/mgen.0.000495
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