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Volume 56, Issue 5

A peptide mimetic of the mycobacterial mannosylated lipoarabinomannan: characterization and potential applications Free

Abstract

Mannosylated lipoarabinomannan (ManLAM), a complex lipoglycan, is a major component of , the agent of tuberculosis (TB), and is an antigen used for serological diagnosis of TB. Screening random phage-display peptide libraries with anti-ManLAM mAb CS40 for peptide epitope mimics (mimotopes) led to the isolation of a panel of peptides. One of these peptides (B11) was characterized as a ManLAM mimotope: it bound the anti-ManLAM CS40 mAb and competed with ManLAM for antibody binding. Mice immunized with keyhole limpet haemocyanin-conjugated B11 peptide in a proper adjuvant developed antibodies that recognized ManLAM. Competition experiments demonstrated that the B11 peptide inhibited binding of mAb CS40 to ManLAM in a concentration-dependent manner. The data indicated that the affinity of CS40 mAb to B11 ( 1.33×10) is higher than its affinity to ManLAM ( 3.00×10). The sera of TB patients, as well as the sera of mice experimentally infected with , contained significant levels of antibodies that recognized both the B11 peptide and ManLAM. The specificity and sensitivity of the ELISA B11-based test were similar to those of the ELISA ManLAM-based test, indicating that the B11 antigen could be a good substitute for ManLAM serology for the diagnosis of TB.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): i.p., intraperitoneally , KLH, keyhole limpet haemocyanin , ManLAM, mannosylated lipoarabinomannan , MPL-TDM, monophosphoryl lipid A+trehalose dimycolate , s.c., subcutaneously , SPR, surface plasmon resonance and TB, tuberculosis
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2007-05-01
2024-04-19
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References

  1. Adamczyk M., Moore J. A., Yu Z. 2000; Application of surface plasmon resonance toward studies of low-molecular-weight antigen–antibody binding interactions. Methods 20:319–328 [CrossRef]
     [Google Scholar]
  2. Antunes A., Nina J., David S. 2002; Serological screening for tuberculosis in the community: an evaluation of the Mycodot procedure in an African population with high HIV-2 prevalence (Republic of Guinea-Bissau). Res Microbiol 153:301–305 [CrossRef]
     [Google Scholar]
  3. Beninati C., Teti G. 2000; A meeting of mimics. Trends Microbiol 8:492–493 [CrossRef]
     [Google Scholar]
  4. Briken V., Porcelli S. A., Besra G. S., Kremer L. 2004; Mycobacterial lipoarabinomannan and related lipoglycans: from biogenesis to modulation of the immune response. Mol Microbiol 53:391–403 [CrossRef]
     [Google Scholar]
  5. Castanon-Arreola M., Lopez-Vidal Y. 2004; A second-generation anti TB vaccine is long overdue. Ann Clin Microbiol Antimicrob 3:10 [CrossRef]
     [Google Scholar]
  6. Chan E. D., Heifets L., Iseman M. D. 2000; Immunologic diagnosis of tuberculosis: a review. Tuber Lung Dis 80:131–140 [CrossRef]
     [Google Scholar]
  7. Charalambous B. M., Feavers I. M. 2000; Peptide mimics elicit antibody responses against the outer-membrane lipooligosaccharide of group B Neisseria meningitidis . FEMS Microbiol Lett 191:45–50 [CrossRef]
     [Google Scholar]
  8. Chatterjee D., Lowell K., Rivoire B., McNeil M. R., Brennan P. J. 1992; Lipoarabinomannan of Mycobacterium tuberculosis . Capping with mannosyl residues in some strains. J Biol Chem 267:6234–6239
     [Google Scholar]
  9. Ciesielski S. D. 1995; BCG vaccination and the PPD test: what the clinician needs to know. J Fam Pract 40:76–80
     [Google Scholar]
  10. Enshell-Seijffers D. J. G. 2002; Phage-display selection and analysis of Ab-binding epitopes. In Current Protocols in Immunology , unit 6.8p. 9.8.1 Edited by Coligan J. E., Kruisbeek A. M., Margulies D. H., Shevach E. M., Strober W. New York: Wiley;
     [Google Scholar]
  11. Enshell-Seijffers D., Smelyanski L., Gershoni J. M. 2001; The rational design of a ‘type 88’ genetically stable peptide display vector in the filamentous bacteriophage fd. Nucleic Acids Res 29:E50 [CrossRef]
     [Google Scholar]
  12. Enshell-Seijffers D., Denisov D., Groisman B., Smelyanski L., Meyuhas R., Gross G., Denisova G., Gershoni J. M. 2003; The mapping and reconstitution of a conformational discontinuous B-cell epitope of HIV-1. J Mol Biol 334:87–101 [CrossRef]
     [Google Scholar]
  13. Fuchs S., Kasher R., Balass M., Scherf T., Harel M., Fridkin M., Sussman J. L., Katchalski-Katzir E. 2003; The binding site of acetylcholine receptor: from synthetic peptides to solution and crystal structure. Ann N Y Acad Sci 998:93–100 [CrossRef]
     [Google Scholar]
  14. Glatman-Freedman A., Casadevall A., Dai Z., Jacobs W. R. Jr, Li A., Morris S. L., Navoa J. A., Piperdi S., Robbins J. B. other authors 2004; Antigenic evidence of prevalence and diversity of Mycobacterium tuberculosis arabinomannan. J Clin Microbiol 42:3225–3231 [CrossRef]
     [Google Scholar]
  15. Grothaus M. C., Srivastava N., Smithson S. L., Kieber-Emmons T., Williams D. B., Carlone G. M., Westerink M. A. 2000; Selection of an immunogenic peptide mimic of the capsular polysaccharide of Neisseria meningitidis serogroup A using a peptide display library. Vaccine 18:1253–1263 [CrossRef]
     [Google Scholar]
  16. Hamasur B., Kallenius G., Svenson S. B. 1999; Synthesis and immunologic characterisation of Mycobacterium tuberculosis lipoarabinomannan specific oligosaccharide-protein conjugates. Vaccine 17:2853–2861 [CrossRef]
     [Google Scholar]
  17. Hamasur B., Bruchfeld J., Haile M., Pawlowski A., Bjorvatn B., Kallenius G., Svenson S. B. 2001; Rapid diagnosis of tuberculosis by detection of mycobacterial lipoarabinomannan in urine. J Microbiol Methods 45:41–52 [CrossRef]
     [Google Scholar]
  18. Hetland G., Wiker H. G., Hogasen K., Hamasur B., Svenson S. B., Harboe M. 1998; Involvement of antilipoarabinomannan antibodies in classical complement activation in tuberculosis. Clin Diagn Lab Immunol 5:211–218
     [Google Scholar]
  19. Honma K., Kato T., Okuda K. 1999; Salivary immunoglobulin A production against a synthetic oligopeptide antigen of Actinobacillus actinomycetemcomitans fimbriae. Oral Microbiol Immunol 14:288–292 [CrossRef]
     [Google Scholar]
  20. Hovav A. H., Mullerad J., Davidovitch L., Fishman Y., Bigi F., Cataldi A., Bercovier H. 2003; The Mycobacterium tuberculosis recombinant 27-kilodalton lipoprotein induces a strong Th1-type immune response deleterious to protection. Infect Immun 71:3146–3154 [CrossRef]
     [Google Scholar]
  21. Kaur D., Lowary T. L., Vissa V. D., Crick D. C., Brennan P. J. 2002; Characterization of the epitope of anti-lipoarabinomannan antibodies as the terminal hexaarabinofuranosyl motif of mycobacterial arabinans. Microbiology 148:3049–3057
     [Google Scholar]
  22. Kieber-Emmons T., Luo P., Qiu J., Chang T. Y., O I., Blaszczyk-Thurin M., Steplewski Z. 1999; Vaccination with carbohydrate peptide mimotopes promotes anti-tumor responses. Nat Biotechnol 17:660–665 [CrossRef]
     [Google Scholar]
  23. Leenaars M., Koedam M. A., Hendriksen C. F., Claassen E. 1998; Immune responses and side effects of five different oil-based adjuvants in mice. Vet Immunol Immunopathol 61:291–304 [CrossRef]
     [Google Scholar]
  24. Maitta R. W., Datta K., Lees A., Belouski S. S., Pirofski L. A. 2004; Immunogenicity and efficacy of Cryptococcus neoformans capsular polysaccharide glucuronoxylomannan peptide mimotope-protein conjugates in human immunoglobulin transgenic mice. Infect Immun 72:196–208 [CrossRef]
     [Google Scholar]
  25. Montalto M. C., Collard C. D., Buras J. A., Reenstra W. R., McClaine R., Gies D. R., Rother R. P., Stahl G. L. 2001; A keratin peptide inhibits mannose-binding lectin. J Immunol 166:4148–4153 [CrossRef]
     [Google Scholar]
  26. Navoa J. A., Laal S., Pirofski L. A., McLean G. R., Dai Z., Robbins J. B., Schneerson R., Casadevall A., Glatman-Freedman A. 2003; Specificity and diversity of antibodies to Mycobacterium tuberculosis arabinomannan. Clin Diagn Lab Immunol 10:88–94
     [Google Scholar]
  27. Ochsenbein A. F., Fehr T., Lutz C., Suter M., Brombacher F., Hengartner H., Zinkernagel R. M. 1999; Control of early viral and bacterial distribution and disease by natural antibodies. Science 286:2156–2159 [CrossRef]
     [Google Scholar]
  28. Park I., Choi I. H., Kim S. J., Shin J. S. 2004; Peptide mimotopes of Neisseria meningitidis group B capsular polysaccharide. Yonsei Med J 45:755–758 [CrossRef]
     [Google Scholar]
  29. Prinz D. M., Smithson S. L., Westerink M. A. 2004; Two different methods result in the selection of peptides that induce a protective antibody response to Neisseria meningitidis serogroup C. J Immunol Methods 285:1–14 [CrossRef]
     [Google Scholar]
  30. Prinzis S., Chatterjee D., Brennan P. J. 1993; Structure and antigenicity of lipoarabinomannan from Mycobacterium bovis BCG. J Gen Microbiol 139:2649–2658 [CrossRef]
     [Google Scholar]
  31. Ratanasuwan W., Kreiss J. K., Nolan C. M., Schaeffler B. A., Suwanagool S., Tunsupasawasdikul S., Chuchottaworn C., Dejsomritrutai W., Foy H. M. 1997; Evaluation of the MycoDot test for the diagnosis of tuberculosis in HIV seropositive and seronegative patients. Int J Tuberc Lung Dis 1:259–264
     [Google Scholar]
  32. Shin J. S., Yu J., Lin J., Zhong L., Bren K. L., Nahm M. H. 2002; Peptide mimotopes of pneumococcal capsular polysaccharide of 6B serotype: a peptide mimotope can bind to two unrelated antibodies. J Immunol 168:6273–6278 [CrossRef]
     [Google Scholar]
  33. Tailleux L., Schwartz O., Herrmann J. L., Pivert E., Jackson M., Amara A., Legres L., Dreher D., Nicod L. P. other authors 2003; DC-SIGN is the major Mycobacterium tuberculosis receptor on human dendritic cells. J Exp Med 197:121–127
     [Google Scholar]
  34. Tang S. S., Tan W. S., Devi S., Wang L. F., Pang T., Thong K. L. 2003; Mimotopes of the Vi antigen of Salmonella enterica serovar typhi identified from phage display peptide library. Clin Diagn Lab Immunol 10:1078–1084
     [Google Scholar]
  35. Taouji S., Nomura I., Giguere S., Tomomitsu S., Kakuda T., Ganne V., Takai S. 2004; Immunogenicity of synthetic peptides representing linear B-cell epitopes of VapA of Rhodococcus equi . Vaccine 22:1114–1123 [CrossRef]
     [Google Scholar]
  36. Wan Y., Wu Y., Bian J., Wang X. Z., Zhou W., Jia Z. C., Tan Y., Zhou L. 2001; Induction of hepatitis B virus-specific cytotoxic T lymphocytes response in vivo by filamentous phage display vaccine. Vaccine 19:2918–2923 [CrossRef]
     [Google Scholar]
  37. Westerink M. A., Giardina P. C., Apicella M. A., Kieber-Emmons T. 1995; Peptide mimicry of the meningococcal group C capsular polysaccharide. Proc Natl Acad Sci U S A 92:4021–4025 [CrossRef]
     [Google Scholar]
  38. Youn J. H., Myung H. J., Liav A., Chatterjee D., Brennan P. J., Choi I. H., Cho S. N., Shin J. S. 2004; Production and characterization of peptide mimotopes of phenolic glycolipid-I of Mycobacterium leprae . FEMS Immunol Med Microbiol 41:51–57 [CrossRef]
     [Google Scholar]
  39. Young A. C., Valadon P., Casadevall A., Scharff M. D., Sacchettini J. C. 1997; The three-dimensional structures of a polysaccharide binding antibody to Cryptococcus neoformans and its complex with a peptide from a phage display library: implications for the identification of peptide mimotopes. J Mol Biol 274:622–634 [CrossRef]
     [Google Scholar]
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A peptide mimetic of the mycobacterial mannosylated lipoarabinomannan: characterization and potential applications
J. Med. Microbiol. 56, 579 (2007); https://doi.org/10.1099/jmm.0.46920-0
/content/journal/jmm/10.1099/jmm.0.46920-0
/content/journal/jmm/10.1099/jmm.0.46920-0
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