1887

Abstract

This study assessed the performance of a rapid, low-cost, colorimetric method, the resazurin microtitre assay (REMA) plate method, for the detection of resistance to isoniazid and rifampicin in 136 clinical isolates of from two hospitals in Algiers. MICs were determined and the results were compared with those obtained with the conventional proportion method on Löwenstein–Jensen medium. Excellent results were obtained for the REMA plate method, with a sensitivity of 100 % for both isoniazid and rifampicin and a specificity of 98.3 and 99.2 %, respectively. The REMA plate method appears to be a reliable method for the rapid determination of multidrug-resistant tuberculosis and is a good alternative for use in resource-limited countries such as Algeria.

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2006-07-01
2024-03-19
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References

  1. Barnes P. F., Lakey D. L., Burman W. J. 2002; Tuberculosis in patients with HIV infection. Infect Dis Clin North Am 16:107–126 [CrossRef]
    [Google Scholar]
  2. Canetti G., Froman S., Grosset J., Hauduroy P., Langerova M., Mahler H. T., Meissner G., Mitchison D. A., Sula L. 1963; Mycobacteria: laboratory methods for testing drug sensitivity and resistance. Bull World Health Organ 29:565–578
    [Google Scholar]
  3. Canetti G., Fox W., Khomenko A., Mahler H. T., Menon N. K., Mitchison D. A., Rist N., Smelev N. A. 1969; Advances in techniques of testing mycobacterial drug sensitivity, and the use of sensitivity tests in tuberculosis control programmes. Bull World Health Organ 41:21–43
    [Google Scholar]
  4. Corbett E. L., Watt C. J., Walker N., Maher D., Williams B. G., Raviglione M. C., Dye C. 2003; The growing burden of tuberculosis: global trends and interactions with the HIV epidemic. Arch Intern Med 163:1009–1021 [CrossRef]
    [Google Scholar]
  5. De Beenhouwer H., Lhiang Z., Jannes G., Mijs W., Machtelinckx L., Rossau R., Traore H., Portaels F. 1995; Rapid detection of rifampicin resistance in sputum and biopsy specimens from tuberculosis patients by PCR and line probe assay. Tuber Lung Dis 76:425–430 [CrossRef]
    [Google Scholar]
  6. Frieden T. R., Sterling T. R., Munsiff S. S., Watt C. J., Dye C. 2003; Tuberculosis. Lancet 362:887–899 [CrossRef]
    [Google Scholar]
  7. Gabrielson J., Hart M., Jarelöv A., Kühn I., McKenzie D., Möllby R. 2002; Evaluation of redox indicators and the use of digital scanners and spectrophotometer for quantification of microbial growth in microplates. J Microbiol Methods 50:63–73 [CrossRef]
    [Google Scholar]
  8. Goloubeva V., Lecocq M., Lassowsky P., Mathys F., Portaels F., Bastian I. 2001; evaluation of mycobacteria growth indicator tube for direct and indirect drug susceptibility testing of Mycobacterium tuberculosis from respiratory specimens in a Siberian prison hospital. J Clin Microbiol 39:1501–1505 [CrossRef]
    [Google Scholar]
  9. Iseman M. D. 1998; MDR-TB and the developing world – a problem no longer to be ignored: the WHO announces ‘DOTS Plus' strategy. Int J Tuberc Lung Dis 2:867
    [Google Scholar]
  10. Kent P. T., Kubica G. P. 1985 Public Health Mycobacteriology. A Guide for the Level III Laboratory Atlanta, GA: US Department of Health and Human Services, Centers for Disease Control and Prevention;
    [Google Scholar]
  11. Lemus D., Martin A., Montoro E., Portaels F., Palomino J. C. 2004; Rapid alternative methods for detection of rifampicin resistance in Mycobacterium tuberculosis . J Antimicrob Chemother 54:130–133 [CrossRef]
    [Google Scholar]
  12. Martin A., Camacho M., Portaels F., Palomino J. C. 2003; Resazurin microtiter assay plate testing of Mycobacterium tuberculosis susceptibilities to second-line drugs: rapid, simple, and inexpensive method. Antimicrob Agents Chemother 47:3616–3619 [CrossRef]
    [Google Scholar]
  13. Montoro E., Lemus D., Echemendia M., Martin A., Portaels F., Palomino J. C. 2005; Comparative evaluation of nitrate reductase assay, the MTT test, and the resazurin microtitre assay for drug susceptibility testing of clinical isolates of Mycobacterium tuberculosis . J Antimicrob Chemother 55:500–505 [CrossRef]
    [Google Scholar]
  14. Moore A. V., Kirk S. M., Callister S. M., Mazurek G. H., Schell R. F. 1999; Safe determination of susceptibility of Mycobacterium tuberculosis to antimicrobial agent by flow cytometry. J Clin Microbiol 37:479–483
    [Google Scholar]
  15. Nachamkin I., Kang C., Weinstein M. P. 1997; Detection of resistance to isoniazid, rifampicin, and streptomycin in clinical isolates of Mycobacterium tuberculosis by molecular methods. Clin Infect Dis 24:894–900 [CrossRef]
    [Google Scholar]
  16. Norden M. A., Kurzynsky T. A., Bownds S. E., Callister S. M., Shell R. F. 1995; Rapid susceptibility testing of Mycobacterium tuberculosis (H37Ra) by flow cytometry. J Clin Microbiol 33:1231–1237
    [Google Scholar]
  17. Palomino J. C., Traore H., Fissette K., Portaels F. 1999; Evaluation of Mycobacteria Growth Indicator Tube (MGIT) for drug susceptibility testing of Mycobacterium tuberculosis . Int J Tuberc Lung Dis 3:344–348
    [Google Scholar]
  18. Palomino J. C., Martin A., Camacho M., Guerra H., Swings J., Portaels F. 2002; Resazurin microtiter assay plate: simple and inexpensive method for detection of drug resistance in Mycobacterium tuberculosis . Antimicrob Agents Chemother 46:2720–2722 [CrossRef]
    [Google Scholar]
  19. Palomino J. C., Martin A., Portaels F. 2004; Rapid colorimetric methods for the determination of drug resistance in Mycobacterium tuberculosis . Res Adv Antimicrob Agents Chemother 4:29–38
    [Google Scholar]
  20. Roberts G. D., Goodman N. L., Heifets L., Larsh H. W., Lindner T. H., McClatchy J. K., McGinnis M. R., Siddiqi S. H., Wright P. 1983; Evaluation of the BACTEC radiometric method for recovery of mycobacteria and drug susceptibility testing of Mycobacterium tuberculosis from acid-fast smear-positive specimens. J Clin Microbiol 18:689–696
    [Google Scholar]
  21. Seung K. J., Gelmanova I. E., Peremitin G. G., Golubchikova V. T., Pavlova V. E., Sirotkina O. B., Yanova G. V., Strelis A. K. 2004; The effect of initial drug resistance on treatment response and acquired drug resistance during standardized short-course chemotherapy for tuberculosis. Clin Infect Dis 39:1321–1328 [CrossRef]
    [Google Scholar]
  22. Siddiqi S. H., Libonati J. P., Middlebrook G. 1981; Evaluation of rapid radiometric method for drug susceptibility testing of Mycobacterium tuberculosis . J Clin Microbiol 13:908–912
    [Google Scholar]
  23. Símboli N., Takiff H., McNerney R., López B., Martin A., Palomino J. C., Barrera L., Ritacco V. 2005; In-house phage amplification assay is a sound alternative for detection of rifampin-resistant tuberculosis in low-resource settings. Antimicrob Agents Chemother 49:425–427 [CrossRef]
    [Google Scholar]
  24. Wanger M., Mills K. 1996; Testing of Mycobacterium tuberculosis susceptibility to ethambutol, isoniazid, rifampin and streptomycin by using Etest. J Clin Microbiol 34:1672–1676
    [Google Scholar]
  25. WHO 2004 Anti-tuberculosis Drug Resistance in the World , Report No. 3. WHO/HTM/TB/2004.343. Geneva: World Health Organization;
    [Google Scholar]
  26. WHO 2005; Global Tuberculosis Control: Surveillance, Planning, Financing . WHO Report 2005; WHO/HTM/TB/2005.349. Geneva: World Health Organization;
    [Google Scholar]
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