1887

Abstract

Three closely related strains isolated from the same patient harboured , , , , , , and genes. Two of the isolates were recovered after treatment with meropenem and showed resistance to carbapenems. Sequencing of and porin genes of the carbapenem-resistant strains revealed the presence of premature stop codons in both, and OmpK35 and OmpK36 porins were not detected by SDS-PAGE. One carbepenem-resistant strain showed a high amount of LamB protein and did not express OmpK26 porin whereas the other strain expressed OmpK26 but not LamB. The lack of major porins apparently causes changes in the expression of other, specific, porins.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.038083-0
2012-05-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/jmm/61/5/672.html?itemId=/content/journal/jmm/10.1099/jmm.0.038083-0&mimeType=html&fmt=ahah

References

  1. Ardanuy C., Liñares J., Domínguez M. A., Hernández-Allés S., Benedí V. J., Martínez-Martínez L. 1998; Outer membrane profiles of clonally related Klebsiella pneumoniae isolates from clinical samples and activities of cephalosporins and carbapenems. Antimicrob Agents Chemother 42:1636–1640[PubMed]
    [Google Scholar]
  2. Carattoli A., Bertini A., Villa L., Falbo V., Hopkins K. L., Threlfall E. J. 2005; Identification of plasmids by PCR-based replicon typing. J Microbiol Methods 63:219–228 [View Article][PubMed]
    [Google Scholar]
  3. CLSI 2011; Performance Standards for Antimicrobial Disk Susceptibility Testing; Approved Standard, 21st edn. Wayne, PA: Clinical and Laboratory Standards Institute;
  4. Doménech-Sánchez A., Martínez-Martínez L., Hernández-Allés S., del Carmen Conejo M., Pascual A., Tomás J. M., Albertí S., Benedí V. J. 2003; Role of Klebsiella pneumoniae OmpK35 porin in antimicrobial resistance. Antimicrob Agents Chemother 47:3332–3335 [View Article][PubMed]
    [Google Scholar]
  5. Doumith M., Ellington M. J., Livermore D. M., Woodford N. 2009; Molecular mechanisms disrupting porin expression in ertapenem-resistant Klebsiella and Enterobacter spp. clinical isolates from the UK. J Antimicrob Chemother 63:659–667 [View Article][PubMed]
    [Google Scholar]
  6. Ellington M. J., Kistler J., Livermore D. M., Woodford N. 2007; Multiplex PCR for rapid detection of genes encoding acquired metallo-β-lactamases. J Antimicrob Chemother 59:321–322 [View Article][PubMed]
    [Google Scholar]
  7. Elliott E., Brink A. J., van Greune J., Els Z., Woodford N., Turton J., Warner M., Livermore D. M. 2006; In vivo development of ertapenem resistance in a patient with pneumonia caused by Klebsiella pneumoniae with an extended-spectrum β-lactamase. Clin Infect Dis 42:e95–e98 [View Article][PubMed]
    [Google Scholar]
  8. Findlay J., Hamouda A., Dancer S. J., Amyes S. G. 2012; Rapid acquisition of decreased carbapenem susceptibility in a strain of Klebsiella pneumoniae arising during meropenem therapy. Clin Microbiol Infect 18:140–146 [View Article][PubMed]
    [Google Scholar]
  9. García-Sureda L., Juan C., Doménech-Sánchez A., Albertí S. 2011a; Role of Klebsiella pneumoniae LamB porin in antimicrobial resistance. Antimicrob Agents Chemother 55:1803–1805 [View Article][PubMed]
    [Google Scholar]
  10. García-Sureda L., Doménech-Sánchez A., Juan C., Albertí S. 2011b; KdgM, a novel porin associated to carbapenem resistance in Klebsiella pneumoniae. In: Abstracts of the 21st European Congress of Clinical Microbiology and Infectious Diseases, Milan, Italy, May 2011, P604
    [Google Scholar]
  11. Hernández-Allés S., Albertí S., Alvarez D., Doménech-Sánchez A., Martínez-Martínez L., Gil J., Tomás J. M., Benedí V. J. 1999; Porin expression in clinical isolates of Klebsiella pneumoniae. Microbiology 145:673–679 [View Article][PubMed]
    [Google Scholar]
  12. Hong S. G., Hong S. K., Huh J. Y., Kang M. S. 2010; Multiplex PCR for rapid detection of genes encoding class A carbapenemases. In: Abstracts of the 20th European Congress of Clinical Microbiology and Infectious Diseases, Vienna, Austria, April 2010, 6, S552
    [Google Scholar]
  13. Kaczmarek F. M., Dib-Hajj F., Shang W., Gootz T. D. 2006; High-level carbapenem resistance in a Klebsiella pneumoniae clinical isolate is due to the combination of blaACT-1 β-lactamase production, porin OmpK35/36 insertional inactivation, and down-regulation of the phosphate transport porin phoE. Antimicrob Agents Chemother 50:3396–3406 [View Article][PubMed]
    [Google Scholar]
  14. Lee C. H., Chu C., Liu J. W., Chen Y. S., Chiu C. J., Su L. H. 2007; Collateral damage of flomoxef therapy: in vivo development of porin deficiency and acquisition of blaDHA-1 leading to ertapenem resistance in a clinical isolate of Klebsiella pneumoniae producing CTX-M-3 and SHV-5 β-lactamases. J Antimicrob Chemother 60:410–413 [View Article][PubMed]
    [Google Scholar]
  15. Martínez-Martínez L., Conejo M. C., Pascual A., Hernández-Allés S., Ballesta S., Ramírez De Arellano-Ramos E., Benedí V. J., Perea E. J. 2000; Activities of imipenem and cephalosporins against clonally related strains of Escherichia coli hyperproducing chromosomal β-lactamase and showing altered porin profiles. Antimicrob Agents Chemother 44:2534–2536 [View Article][PubMed]
    [Google Scholar]
  16. Martínez-Martínez L., Pascual A., Conejo M. C., García I., Joyanes P., Doménech-Sánchez A., Benedí V. J. 2002; Energy-dependent accumulation of norfloxacin and porin expression in clinical isolates of Klebsiella pneumoniae and relationship to extended-spectrum β-lactamase production. Antimicrob Agents Chemother 46:3926–3932 [View Article][PubMed]
    [Google Scholar]
  17. Pérez-Pérez F. J., Hanson N. D. 2002; Detection of plasmid-mediated AmpC beta-lactamase genes in clinical isolates by using multiplex PCR. J Clin Microbiol 40:2153–2162 [View Article][PubMed]
    [Google Scholar]
  18. Ruiz E., Rojo-Bezares B., Sáenz Y., Olarte I., Esteban I., Rocha-Gracia R., Zarazaga M., Torres C. 2010; Outbreak caused by a multi-resistant Klebsiella pneumoniae strain of new sequence type ST341 carrying new genetic environments of aac(6′)-Ib-cr and qnrS1 genes in a neonatal intensive care unit in Spain. Int J Med Microbiol 300:464–469 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.038083-0
Loading
/content/journal/jmm/10.1099/jmm.0.038083-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error